Evidence map›Paper›PMID 41868125›Full record

ArticleFrontiers in pharmacology2026

Shikonin inhibits MRSA biofilm formation to alleviate periprosthetic joint infection.

Shangyi Liu, Haoran Zhang, Xi Zheng, Xiaoqin Mou, Zhongbao Wu, Lili Zou, Kangquan Shou, Xiaowen Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in pharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Shangyi Liu *Department of Orthopedics, The First College of Clinical Medical Science and Yichang Central People's Hospital, China Three Gorges University, Yichang, China.
Haoran Zhang *Hubei Key Laboratory of Tumor Microenvironment and Immunotherapy and College of Basic Medical Sciences, China Three Gorges University, Yichang, China.
Xi ZhengHubei Key Laboratory of Tumor Microenvironment and Immunotherapy and College of Basic Medical Sciences, China Three Gorges University, Yichang, China.
Xiaoqin MouHubei Key Laboratory of Tumor Microenvironment and Immunotherapy and College of Basic Medical Sciences, China Three Gorges University, Yichang, China.
Zhongbao WuHubei Key Laboratory of Tumor Microenvironment and Immunotherapy and College of Basic Medical Sciences, China Three Gorges University, Yichang, China.
Lili ZouHubei Key Laboratory of Tumor Microenvironment and Immunotherapy and College of Basic Medical Sciences, China Three Gorges University, Yichang, China.
Kangquan ShouDepartment of Orthopedics, The First College of Clinical Medical Science and Yichang Central People's Hospital, China Three Gorges University, Yichang, China.
Xiaowen LiuDepartment of Orthopedics, The First College of Clinical Medical Science and Yichang Central People's Hospital, China Three Gorges University, Yichang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: To alleviate periprosthetic joint infection (PJI) with methicillin-resistant Methods: The rat model of periprosthetic joint infection was established, utilizing techniques such as scanning electron microscopy and pathology test to evaluate the MRSA inhibitory of bacterial load and biofilm formation effects of SKN. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) experiments were conducted to assess the antibacterial activity of SKN. The crystal violet staining method was employed to evaluate the effects of SKN on MRSA biofilm formation and eradication. Transcriptomic and amino acid metabolomics analyses were used to investigate the mechanism of SKN inhibition in MRSA biofilm formation. Total thiol detection was used to assess the impact of SKN on the intracellular cysteine levels in MRSA. Finally, MIC and crystal violet staining were used to evaluate the antibacterial effects and biofilm eradication efficacy of SKN against clinical MRSA strains. Results: Conclusion: SKN inhibits MRSA by suppressing biofilm formation, effectively alleviating periprosthetic joint infection by MRSA, and the mechanism of SKN antibacterial activity may be related to regulate the cysteine metabolism in MRSA.

Indexed as

biofilm formationcysteine metabolismmethicillin-resistant Staphylococcus aureus (MRSA)periprosthetic joint infection (PJI)shikonin (SKN)

Identifiers

PMID41868125
PMCPMC13002819

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.