ArticleFrontiers in pediatrics2026
Comparative evaluation of RNA isothermal amplification-gold probe lateral flow assay and targeted next-generation sequencing for the detection of
Article in Frontiers in pediatrics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
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Authors and funding
6 authors.
Funding
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Abstract
Objectives: This study aimed to compare the positive detection rates of RNA Isothermal Amplification-Gold Probe Lateral Flow Technology (RGT) and Targeted Next-Generation Sequencing (tNGS) for Methods: Clinical and laboratory data of pediatric patients with ARIs undergoing concurrent RGT and tNGS testing (Jan-Sep 2024) were collected. McNemar's test compared detection rates, and Cohen's kappa coefficient assessed the agreement of the result of between the two methods. Results: For detecting MP and Influenza B virus, tNGS showed a significantly higher positivity rate than RGT. However, there is no difference between tNGS and RGT in the positive detection rate of Influenza A. The agreement between tNGS and RGT was good for MP detection, but only moderate for Influenza A/B virus detection. Conclusions: tNGS offers high-throughput, high-sensitivity screening, while RGT is rapid, user-friendly, cost-effective and superior for identifying active infections and monitoring treatment responses. Optimal method selection is dependent on clinical scenarios and diagnostic priorities.
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