Evidence map›Paper›PMID 41866455›Full record

ArticleScience China. Life sciences2026

Ultra-fast generation of all-female grass carp via transplantation of female germline stem cells into zebrafish.

Ding Ye, Yongkang Hao, Junwen Zhu, Chaofan Wang, Linglu Li, Yongming Li, Yi-Xuan Tu, Zhiqin Ren, Houpeng Wang, Zhengfang Chen and 3 more

Abstract read
PubMed Publisher
In one paragraph

Article in Science China. Life sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Germ cell manipulation in farmed fishes.The Journal of reproduction and development · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Ding Ye *State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Yongkang Hao *State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Junwen Zhu *State Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Chaofan WangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Linglu LiState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Yongming LiState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Yi-Xuan TuState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Zhiqin RenState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Houpeng WangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Zhengfang ChenState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Mudan HeState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.
Xiaosi WangState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. wangxs@ihb.ac.cn.
Yonghua SunState Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture, Hubei Hongshan Laboratory, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China. yhsun@ihb.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Surrogate reproduction offers a promising biotechnological approach for accelerating aquaculture breeding. Grass carp (Ctenopharyngodon idellus), a key freshwater species, faces significant constraints in breeding due to its prolonged sexual maturation of 5 years and huge body size. Here, we establish an ultra-fast breeding strategy to generate all-female grass carp within six months via surrogate production in tiny laboratory fish, zebrafish (Danio rerio). We identify and isolate female GSCs from juvenile ovaries of 3-month-old grass carp. Three months after transplantation into zebrafish larvae, the donor-derived female GSCs undergo ultra-fast spermatogenesis and differentiate into functional grass carp sperm carrying X chromosomes. Fertilization of wild-type grass carp eggs with this sperm yields all-female offspring. This work demonstrates that fish female GSCs with XX chromosomes can differentiate into functional sperm in a short time in zebrafish gonadal somatic niche, opening a new avenue for precision breeding and sex control in aquaculture species.

Indexed as

CarpsStem Cell TransplantationZebrafishAnimalsAquacultureBreedingCell DifferentiationFemaleMaleOvarySpermatogenesisSpermatozoaX Chromosomefemale germline stem cellgrass carpmaturation periodsurrogate reproductionzebrafish

Identifiers

PMID41866455

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.