Evidence map›Paper›PMID 41866194›Full record

ArticleThe Journal of reproduction and development2026

Sex determination and genetic screening of equine embryos with whole genome amplification and real-time PCR.

Gus R McFarlane, Elizabeth Backhouse, Agustin Ruiz, Brendon A O'Rourke, Jenin V Cortez, Christopher G Grupen

Abstract read
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In one paragraph

Article in The Journal of reproduction and development, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Gus R McFarlaneNSW Department of Primary Industries and Regional Development, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, Australia.
Elizabeth BackhouseSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia.
Agustin RuizSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia.
Brendon A O'RourkeNSW Department of Primary Industries and Regional Development, Elizabeth Macarthur Agricultural Institute, Menangle, NSW, Australia.
Jenin V CortezSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia.
Christopher G GrupenSydney School of Veterinary Science, Faculty of Science, The University of Sydney, Camden, NSW, Australia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Reliable pre-implantation sex determination and genetic screening enables informed embryo transfer decisions in equine breeding while avoiding later interventions. We developed a streamlined workflow that couples rapid whole genome amplification (WGA) with a multiplex real-time PCR targeting ETSTY5 as a Y-specific marker and UBC as an autosomal control. On purified equine DNA, sex was correctly assigned down to 10 pg gDNA and to a single fibroblast cell. Direct testing of embryo biopsies without WGA yielded inconsistent results, whereas introducing a short WGA step produced tight allelic-discrimination clusters and 100% diagnostic calls, including in cloned embryos of known sex. The same WGA product supported targeted genotyping for inherited disease screening of hyperkalemic periodic paralysis (HYPP) and hereditary equine regional dermal asthenia (HERDA) alleles. This WGA plus real-time PCR pipeline supports robust and practical embryo sexing and targeted pre-implantation genetic diagnostics within in vitro produced equine embryo and embryo transfer workflows.

Indexed as

Genetic TestingPreimplantation DiagnosisReal-Time Polymerase Chain ReactionSex Determination AnalysisAnimalsEmbryo, MammalianEmbryo TransferFemaleHorsesMaleEmbryo biopsyEquine embryosGenetic screeningReal-time PCRSex determinationWhole genome amplification

Identifiers

PMID41866194
PMCPMC13441534

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.