Evidence map›Paper›PMID 41865835›Full record

GuidelineCellular and molecular gastroenterology and hepatology2026

Guiding Principles: Reporting Elements for Gastrointestinal Organoid Research.

Kelli L VanDussen, Tatiana A Karakasheva, Maxime M Mahe, Joseph Burclaff, Luis F Arrieta-Viana, Ian Williamson, Heather A McCauley, Andrés J García, Scott T Magness, Kathryn E Hamilton

Abstract readReviewPractice Guideline
In one paragraph

Guideline in Cellular and molecular gastroenterology and hepatology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Early-life colonization withScience (New York, N.Y.) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Kelli L VanDussenDivision of Gastroenterology, Hepatology, and Nutrition, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio; Department of Pediatrics, Cincinnati Children's Hospital Medical Center, University of Cincinnati College of Medicine, Cincinnati, Ohio.
Tatiana A KarakashevaDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania.
Maxime M MaheNantes Université, Inserm, TENS UMR1235, The Enteric Nervous System in Gut and Brain Diseases, IMAD, Nantes, France; Center for Stem Cell and Organoid Medicine, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio.
Joseph BurclaffLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill/North Carolina State University, Chapel Hill, North Carolina; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
Luis F Arrieta-VianaWoodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia.
Ian WilliamsonDepartment of Molecular and Biomedical Sciences, North Carolina State University, Raleigh, North Carolina.
Heather A McCauleyCenter for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina; Department of Cell Biology and Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina.
Andrés J GarcíaWoodruff School of Mechanical Engineering, Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia.
Scott T MagnessLampe Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill/North Carolina State University, Chapel Hill, North Carolina; Center for Gastrointestinal Biology and Disease, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina. Electronic address: magness@med.unc.edu.
Kathryn E HamiltonDivision of Gastroenterology, Hepatology, and Nutrition, Department of Pediatrics, Children's Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania; Institute for Regenerative Medicine, University of Pennsylvania, Philadelphia, Pennsylvania. Electronic address: hamiltonk1@chop.edu.

Funding

PILOT AND FEASIBILITY STUDIESP30DK034987 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ROBERT S. SANDLER · 1985 to 2026
$30.5M
Understanding Sensorimotor Control Through Realistic Neuro-Biomechanical SimulationU01NS136507 · NINDS · HARVARD UNIVERSITY · PI Bingni Wen Brunton, Bence P Olveczky · 2024 to 2026
$7.1M
DEVELOPMENT OF HUMAN INTESTINAL SIMULACRAR01DK109559 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI ALLBRITTON, NANCY L., BULTMAN, SCOTT J · 2015 to 2019
$5.2M
Defining post-transcriptional regulons in intestinal epithelial regenerationR01DK124369 · NIDDK · CHILDREN'S HOSP OF PHILADELPHIA · PI HAMILTON, KATHRYN ELIZABETH · 2020 to 2024
$2.6M
Contributions of the enterocyte brush border to intestinal health and diseaseR01DK132043 · NIDDK · CINCINNATI CHILDRENS HOSP MED CTR · PI Kelli Lynn VanDussen · 2023 to 2026
$1.9M
Autophagy, injury, and plasticity in the intestinal regenerative processR01DK142682 · NIDDK · CHILDREN'S HOSP OF PHILADELPHIA · PI Kathryn Elizabeth Hamilton · 2025 to 2026
$1.7M
Genetic Control of Intestinal Epithelial Stem Cell Maintenance & DifferentiationR01DK091427 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI MAGNESS, SCOTT T · 2011 to 2015
$1.6M
Genetic regulation of active and reserve intestinal stem cell statesR01DK115806 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI MAGNESS, SCOTT T · 2019 to 2022
$1.5M
Quantifying Enteric Metabolism of Branched-chain Amino Acids in Relation to Other Dietary and Microbiota NutrientsR00DK132554 · NIDDK · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI Ian Andrew Williamson · 2024 to 2026
$747k
Enteroendocrine Regulation of Intestinal MetabolismK01DK125341 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI MCCAULEY, HEATHER A · 2021 to 2025
$728k
Transcriptional regulation of mitochondrial metabolism in human intestinal stem cellsK01DK140610 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Joseph R Burclaff · 2025 to 2026
$337k
Enteroendocrine regulation of intestinal barrier functionR03DK142839 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Heather A McCauley · 2026 to 2026
$113k
NIDDK NIH HHS K01 DK125341NIDDK NIH HHS K01 DK140610NIDDK NIH HHS P30 DK034987NIDDK NIH HHS R00 DK132554NIDDK NIH HHS R01 DK091427NIDDK NIH HHS R01 DK109559NIDDK NIH HHS R01 DK115806NIDDK NIH HHS R01 DK124369NIDDK NIH HHS R01 DK132043NIDDK NIH HHS R01 DK142682NIDDK NIH HHS R03 DK142839NINDS NIH HHS U01 NS136507
6 · The paper itself

Abstract

Gastrointestinal (GI) organoids are now widely used for disease modeling, drug discovery, regenerative-, and precision medicine. As publications using GI organoids have increased exponentially, methodological reporting across GI organoid studies remains inconsistent, making results difficult to interpret, compare, and reproduce. To address this, we present community-informed guidance for reporting key experimental details in GI organoid research. Our recommendations were developed by integrating established biomedical reporting frameworks, organoid-focused resources from international organizations, and structured input from the GI organoid community through an expert panel survey. These inputs informed a core set of reporting items, including stem cell source and donor context, media and supplement composition, extracellular matrix type, culture configuration, and other parameters essential for replication. We provide an "Organoid Reporting Toolkit" containing practical checklists and templates intended to help authors, reviewers, editors, and journals. Together, these resources aim to improve transparency and reproducibility, facilitate cross-study comparison, and streamline communication in the GI organoid field while preserving flexibility for methodological innovation.

Indexed as

Biomedical ResearchGastrointestinal TractOrganoidsResearch DesignAnimalsHumansColonoidEnteroidGastrointestinal organoidInduced pluripotent stem cell derived organoidReporting transparency

Identifiers

PMID41865835
PMCPMC13213672

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.