ArticleACS synthetic biology2026
Vacuum and Sonication Treatment Enable Efficient Transient Gene Expression in Various Monocot and Eudicot Plant Seedlings.
Article in ACS synthetic biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed.
- Sentinel plants enable quantitative monitoring of bioavailable nitrate in soils and microbial environments.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Optimized R2 retroelement complexes for DNA insertion into plant genomes.Nature biotechnology · 2026Article
- Strain, procedures, and tools for reproducible genetic transformation and genome editing of the emerging plant model Spirodela polyrhiza.The New phytologist · 2026Article
- Establishing Reagent Testing Platforms for Functional Analyses in Sunflower.Plants (Basel, Switzerland) · 2025Article
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10 authors.
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Abstract
Transient gene expression in intact plants is essential for rapidly addressing biological questions, and the current toolkit can be improved to achieve higher efficiency and a broader range of plant species. Here, we introduce VAST (Vacuum and Sonication-Assisted Transient transformation): a transient transformation method that substantially enhances gene expression efficiency and versatility across diverse monocot and eudicot seedlings. By systematically optimizing plant growth conditions and incorporating vacuum infiltration and sonication pretreatments prior to seedling coculture with
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