Evidence map›Paper›PMID 41864867›Full record

ArticleBMC microbiology2026

Synergistic effects of cellulase and lactic acid bacteria(Pediococcus pentosaceus and Levilactobacillus brevis) on alfalfa silage fermentation and microbial dynamics.

Weiqin Ma, Baochao Bai, Mingjian Liu, Panjie Sheng, Jian Bao, Chaoran Song, Jianliang Liu, Hongwei Ma, Shuai Du, Gentu Ge and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Weiqin MaCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Baochao BaiCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Mingjian LiuCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Panjie ShengCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Jian BaoInner Mongolia Academy of Agriculture & Animal Husbandry Science, Hohhot, Inner Mongolia, China.
Chaoran SongCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Jianliang LiuCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Hongwei MaInner Mongolia Opportunus Eco-Agriculture (Group) Co, Hohhot, Inner Mongolia, China.
Shuai DuCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Gentu GeCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Yushan JiaCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China.
Zhijun WangCollege of Grassland Science, Inner Mongolia Agricultural University, Hohhot, Inner Mongolia, China. zhijunwang321@126.com.

Funding

Research and Demonstration of High-Quality Forage Supply and Efficient Breeding Technology System for Dairy Cows (2025YFHH0210)
6 · The paper itself

Abstract

This study evaluated the effects of Lactic acid bacteria and cellulase, individually and in combination, on fermentation quality and microbial community dynamics of alfalfa silage. Six treatments were tested, including control, cellulase alone and two lactic acid bacteria species (Pediococcus pentosaceus, Levilactobacillus brevis) applied individually or in combination with cellulase. The results showed that Levilactobacillus brevis in combination with cellulase, producing higher lactic acid concentrations, lower pH (< 4.2) to the other treatments. The microbiome analysis revealed that Lactiplantibacillus was dominant, while undesirable bacterium Achromobacter was suppressed. Functional prediction of microbial communities analysis indicated a higher predicted abundance of sequences associated with pyruvate metabolism, glycolysis/gluconeogenesis and starch and sucrose metabolism pathways. These findings provide insights into optimizing alfalfa silage quality through synergistic use of cellulase and lactic acid bacteria silage inoculants with high metabolic stability.

Indexed as

CellulaseLevilactobacillus brevisMedicago sativaPediococcus pentosaceusSilageFermentationHydrogen-Ion ConcentrationLactic AcidMicrobiotaCellulaseLactic AcidAdditivesAlfalfaFermentation qualityMicrobial community

Identifiers

PMID41864867
PMCPMC13126710

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.