ArticleJournal of assisted reproduction and genetics2026
Oxygen equilibration dynamics in assisted reproductive technology embryo culture media.
Article in Journal of assisted reproduction and genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
- Update of
Authors and funding
5 authors.
Funding
Abstract
purposeOptimal oxygen saturation is essential for successful in vitro embryo culture in assisted reproductive technology (ART). Reduced oxygen levels (3-8%) improve embryo development by minimizing oxidative stress; however, limited knowledge exists about transient oxygen fluctuations during handling outside hypoxic incubators. This study aimed to quantify oxygen saturation dynamics in embryo culture media under conditions designed to replicate routine ART laboratory workflows.
methodsEmbryo culture media droplets were prepared in room air and overlayed with light or heavy mineral oil. Dishes were equilibrated in a hypoxia chamber (5% O
resultsMedia was allowed to equilibrate from atmospheric to hypoxic conditions for 12 h. Light oil overlays demonstrated faster equilibration (half-life 71 min) compared to heavy oil (half-life 116 min, p < 0.0001). Upon reoxygenation, oxygen saturation rose rapidly, with light oil droplets reoxygenating with a half-time rise of 50 min and heavy oil in 78 min (p < 0.0001).
conclusionIn ART media, hypoxic oxygen equilibration is a gradual process while reoxygenation is rapid. Oil viscosity significantly influences oxygen equilibration dynamics, with light oil permitting faster equilibration and reoxygenation. These findings underscore the importance of minimizing atmospheric exposure during routine handling and highlight the role of overlay oil in reducing transient oxygen fluctuations.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.