Evidence map›Paper›PMID 41862988›Full record

ArticleStem cell research & therapy2026

Rejuvenation of mesenchymal stromal cells via partial reprogramming enables scalable generation of transcriptionally diverse MSC libraries.

Haochen Tu, Aoi Hosaka, Genki Hichiwa, Yayan Wang, Kanako Kazuki, Toshiaki Tabata, Mitsuhiko Osaki, Yuji Nakayama, Iori Kanazawa, Kazuhisa Honma and 6 more

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Article in Stem cell research & therapy, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Haochen TuDepartment of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Aoi HosakaNihon BioData Corporation, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa, 213-0012, Japan.
Genki HichiwaDepartment of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Yayan WangDepartment of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Kanako KazukiChromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Toshiaki TabataPOC Inc., 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Mitsuhiko OsakiChromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Yuji NakayamaDivision of Radioisotope Science, Research Initiative Center, Organization for Research Initiative and Promotion, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Iori KanazawaTrans Chromosomics Inc., 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Kazuhisa HonmaTrans Chromosomics Inc., 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan.
Makoto T KimuraRMDC Inc., 6-17-11 Jingumae, Shibuya-ku, Tokyo, 150-0001, Japan.
Xu GaoDepartment of Biochemistry and Molecular Biology, Harbin Medical University, Harbin, 150081, Heilongjiang, China.
Norichika OgataNihon BioData Corporation, 3-2-1 Sakado, Takatsu-ku, Kawasaki, Kanagawa, 213-0012, Japan.
Satoshi AbeChromosome Engineering Research Center, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. sabe@tottori-u.ac.jp.ORCID 0000-0003-1153-0720
Mitsuo OshimuraPOC Inc., 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. moshimura@trans-chromo.com.ORCID 0000-0003-2640-7039
Yasuhiro KazukiDepartment of Chromosome Biomedical Engineering, Integrated Medical Sciences, Graduate School of Medical Sciences, Tottori University, 86 Nishi-cho, Yonago, Tottori, 683-8503, Japan. kazuki@tottori-u.ac.jp.ORCID 0000-0003-4818-4710

Funding

Exploratory Research Center on Life and Living Systems, National Institutes of Natural Sciences 21-101Japan Agency for Medical Research and Development JP25am0521002JST SPRING JPMJSP2187
6 · The paper itself

Abstract

backgroundMesenchymal stromal cells (MSCs) are widely used in regenerative medicine, but their clinical utility is limited by replicative senescence. Strategies that reverse aging while maintaining MSC identity are urgently needed.

methodsWe developed a non-integrating, temperature-sensitive Sendai virus (SeV)-mediated rejuvenation protocol transiently expressing hTERT, BMI1, and SV40T in human MSCs. Following SeV removal, we evaluated proliferation, telomere length, karyotype stability, transcriptomic reset, producing heterogeneity, and differentiation potential.

resultsRejuvenated MSCs (rej-MSCs) demonstrated extended proliferation beyond 100 days, telomere elongation, and normal karyotypes after SeV clearance. Transcriptomic profiling showed a reset of senescence-associated programs while retaining mesenchymal identity. Functional analyses revealed clone-specific heterogeneity, including HGF-driven angiogenic activity. Multilineage differentiation capacity was preserved across rej-MSCs.

conclusionsThis transient, non-integrating rejuvenation strategy establishes an operational definition of rej-MSCs and provides a transcriptionally diverse and scalable platform for MSC manufacturing and precision therapy design.

Indexed as

Cellular ReprogrammingMesenchymal Stem CellsRejuvenationAntigens, Polyomavirus TransformingCell DifferentiationCell ProliferationCells, CulturedCellular SenescenceHepatocyte Growth FactorHumansPolycomb Repressive Complex 1Sendai virusTelomeraseAntigens, Polyomavirus TransformingBMI1 protein, humanHepatocyte Growth FactorPolycomb Repressive Complex 1TelomeraseTERT protein, humanMesenchymal stromal cellsPartial reprogrammingRegenerative medicineRejuvenationSendai virus

Identifiers

PMID41862988
PMCPMC13126874

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.