Evidence map›Paper›PMID 41862525›Full record

ArticleScientific reports2026

Melanin concentrating hormone regulates bone cell activities and calcium metabolism in regenerating goldfish scales.

Kohei Kuroda, Satsuki Kimura, Kanta Mizusawa, Akiyoshi Takahashi, Yusuke Maruyama, Yoshiaki Tabuchi, Yukihiro Furusawa, Tasuku Hirose, Kaito Hatano, Akihiro Sakatoku and 8 more

Abstract read
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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Kohei Kuroda *Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Noto-cho, Ishikawa, 927-0553, Japan.
Satsuki Kimura *Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Noto-cho, Ishikawa, 927-0553, Japan.
Kanta MizusawaSchool of Marine Biosciences, Kitasato University, Sagamihara, 252-0373, Kanagawa, Japan.
Akiyoshi TakahashiThe Kitasato Institute, Shirokane, Minato-ku, Tokyo, 108-8641, Japan.
Yusuke MaruyamaDepartment of Sport and Wellness, College of Sport and Wellness, Rikkyo University, Niiza, 352-8558, Saitama, Japan.
Yoshiaki TabuchiLife Science Research Center, University of Toyama, Sugitani, Toyama, 930-0194, Japan.
Yukihiro FurusawaDepartment of Pharmaceutical Engineering, Faculty of Engineering, Toyama Prefectural University, Kurokawa, Toyama, 939-0398, Japan.
Tasuku HiroseNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Noto-cho, Ishikawa, 927-0553, Japan.
Kaito HatanoNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Noto-cho, Ishikawa, 927-0553, Japan.
Akihiro SakatokuSchool of Science, Academic Assembly, University of Toyama, Gofuku, Toyama, 930-8555, Japan.
Masato HondaBotanical Garden, Institute of Nature and Environmental Technology, Kanazawa University, Ishikawa, 920-1192, Japan.
Hajime MatsubaraNoto Center for Fisheries Science and Technology, Kanazawa University, Ossaka, Noto-cho, Ishikawa, 927-0552, Japan.
Hiroyuki MishimaDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan.
Naruto YoshidaDepartment of Sport and Wellness, College of Sport and Wellness, Rikkyo University, Niiza, 352-8558, Saitama, Japan.
Ajai K SrivastavDepartment of Zoology, D.D.U. Gorakhpur University, Gorakhpur, 273-009, India.
Jun HirayamaFaculty of Education, Bunkyo University, Koshigaya, 343-8511, Saitama, Japan.
Atsuhiko HattoriDepartment of Sport and Wellness, College of Sport and Wellness, Rikkyo University, Niiza, 352-8558, Saitama, Japan.
Nobuo SuzukiNoto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Noto-cho, Noto-cho, Ishikawa, 927-0553, Japan. nobuos@staff.kanazawa-u.ac.jp.

Funding

Grant-in-Aid for Scientific Research [B] by JSPS 25K03509Grant-in-Aid for Scientific Research [C] by JSPS 22k11823Grant-in-Aid for Scientific Research [C] by JSPS 23K10933JST SPRING JPMJSP2135the cooperative research program of the Institute of Nature and Environmental Technology, Kanazawa University 24024the cooperative research program of the Institute of Nature and Environmental Technology, Kanazawa University 24026the cooperative research program of the Institute of Nature and Environmental Technology, Kanazawa University 24033
6 · The paper itself

Abstract

Previous studies in mice have reported that melanin-concentrating hormone (MCH) regulates bone calcification, an essential process in bone regeneration. However, the mechanisms by which MCH influences the activities of bone cells involved in calcification remain unclear. Goldfish scales, like mammalian bone, consist of osteoblasts, osteoclasts, and a calcified matrix, making regenerating goldfish scales a useful model for studying bone regeneration. In the present study, a single MCH treatment suppressed the expression of genes encoding proteins associated with osteoclast activity in cultured goldfish scales. Moreover, intraperitoneal administration of MCH decreased plasma calcium concentrations and tartrate-resistant acid phosphatase (TRAP) activity, an osteoclast activity marker, in goldfish 24 h after administration. Gene expression analysis suggested that MCH receptor 2 is involved in MCH-mediated regulation of calcification in goldfish scales. In contrast, continuous intraperitoneal administration of MCH elevated both alkaline phosphatase, an osteoblast marker, and TRAP activities in regenerating scales and resulted in decreased scale calcium levels. These results indicate that a single MCH treatment inhibits osteoclast activity in goldfish scales, leading to reduced plasma calcium levels, whereas prolonged MCH treatment affects both osteoclasts and osteoblasts in regenerating scales, thereby resulting in decreased calcium contents in the scales.

Indexed as

Animal ScalesBone RegenerationCalciumGoldfishHypothalamic HormonesMelaninsOsteoblastsOsteoclastsPituitary HormonesRegenerationAlkaline PhosphataseAnimalsCalcification, PhysiologicReceptors, Pituitary HormoneTartrate-Resistant Acid PhosphataseAlkaline PhosphataseCalciumHypothalamic Hormonesmelanin-concentrating hormonemelanin-concentrating hormone receptorMelaninsPituitary HormonesReceptors, Pituitary HormoneTartrate-Resistant Acid PhosphataseCalcitoninCalcium metabolismFish scalesMelanin-concentrating hormoneOsteoblastsOsteoclastsParathyroid hormone–related protein

Identifiers

PMID41862525
PMCPMC13144606

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.