Evidence map›Paper›PMID 41862150›Full record

ArticleBiochimie2026

A single intra-articular injection of IDO-Gal3 shifts synovial fluid metabolic profiles for up to six weeks in male rats with knee instability.

Folly M Patterson, Carlos J Cruz, Brittany D Partain, Hope Welhaven, Estefania Urrego Hernández, Austen Breland, Jacob L Griffith, Arun Wanchoo, Jonathan O Cooper, Joshua F Yarrow and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biochimie, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Folly M PattersonJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA; Pain Research and Intervention Center of Excellence, Clinical and Translational Science Institute, University of Florida, 2004 Mowry Rd, Gainesville, FL, 32610, USA. Electronic address: f.patterson@ufl.edu.
Carlos J CruzJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA; Pain Research and Intervention Center of Excellence, Clinical and Translational Science Institute, University of Florida, 2004 Mowry Rd, Gainesville, FL, 32610, USA. Electronic address: cruzcarlos@ufl.edu.
Brittany D PartainJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA. Electronic address: brittanypartain0112@gmail.com.
Hope WelhavenDepartment of Chemistry and Biochemistry, Montana State University, 103 Chemistry and Biochemistry Building, Bozeman, MT, 59717, USA. Electronic address: hwelhaven@gmail.com.
Estefania Urrego HernándezJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA; Research Service, Malcolm Randall Department of Veterans Affairs Medical Center, North, FL/South Georgia Veterans Health System, Mail Code 151, 1601 SW Archer Rd, Gainesville, FL, 32608, USA. Electronic address: estefani.urregoh@ufl.edu.
Austen BrelandJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA. Electronic address: abreland@ufl.edu.
Jacob L GriffithJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA. Electronic address: jlgriffith2018@gmail.com.
Arun WanchooJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA. Electronic address: awanchoo@bme.ufl.edu.
Jonathan O CooperJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA. Electronic address: jonathancooper1@ufl.edu.
Joshua F YarrowResearch Service, Malcolm Randall Department of Veterans Affairs Medical Center, North, FL/South Georgia Veterans Health System, Mail Code 151, 1601 SW Archer Rd, Gainesville, FL, 32608, USA; Division of Endocrinology, Diabetes and Metabolism, College of Medicine, University of Florida, 1549 Gale Lemerand Dr, Gainesville, FL, 32610, USA; Brain Rehabilitation Research Center, Malcolm Randall Department of Veterans Affairs Medical Center, North, FL/South Georgia Veterans Health System, 1601 SW Archer Rd, Gainesville, FL, 32608, USA. Electronic address: joshua.yarrow@va.gov.
Ronald K JuneDepartment of Mechanical and Industrial Engineering, Montana State University, 220 Roberts Hall, Bozeman, MT, 59717, USA. Electronic address: rjune@montana.edu.
Benjamin G KeselowskyJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA. Electronic address: bkeselowsky@bme.ufl.edu.
Gregory A HudallaJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA. Electronic address: ghudalla@bme.ufl.edu.
Kyle D AllenJ. Crayton Pruitt Family Department of Biomedical Engineering, University of Florida, 1275 Center Dr, JG56, Gainesville, FL, 32610, USA; Pain Research and Intervention Center of Excellence, Clinical and Translational Science Institute, University of Florida, 2004 Mowry Rd, Gainesville, FL, 32610, USA; Orthopaedics and Sports Medicine Institute, University of Florida, 3450 Hull Rd, Gainesville, FL, 32607, USA. Electronic address: kyle.allen@bme.ufl.edu.

Funding

Research Core (Developmental Research Project Program)P20GM103474 · NIGMS · MONTANA STATE UNIVERSITY - BOZEMAN · PI Ann Therese Bertagnolli · 2012 to 2026
$60.0M
Innervation of the knee and TMJUC2AR082196 · NIAMS · UNIVERSITY OF FLORIDA · PI Kyle D Allen, Alejandro Jose Almarza · 2022 to 2026
$11.2M
the Integrative and Multidisciplinary Pain and Aging Research Training (IMPART) ProgramT32AG049673 · NIA · UNIVERSITY OF FLORIDA · PI FILLINGIM, ROGER B · 2015 to 2024
$3.2M
Treatment of Knee Osteoarthritis via Intra-articular Delivery of an Immunosuppressive EnzymeR01AR079874 · NIAMS · UNIVERSITY OF FLORIDA · PI Kyle D Allen · 2022 to 2026
$3.1M
OA Pathogenesis beyond Cartilage: A preclinical study of the sources of OA painR01AR071431 · NIAMS · UNIVERSITY OF FLORIDA · PI ALLEN, KYLE D · 2018 to 2022
$2.3M
Ultra Performance Liquid Chromatography Tandem Mass Spectrometer for MetabolomicsS10OD028650 · OD · MONTANA STATE UNIVERSITY - BOZEMAN · PI BOTHNER, BRIAN P · 2020 to 2020
$599k
BLRD VA IS1 BX006057NIAMS NIH HHS R01 AR071431NIAMS NIH HHS R01 AR079874NIAMS NIH HHS UC2 AR082196NIA NIH HHS T32 AG049673NIGMS NIH HHS P20 GM103474NIH HHS S10 OD028650
6 · The paper itself

Abstract

Joint metabolism is a potential therapeutic target space for osteoarthritis. We have previously developed a fusion protein of indoleamine 2,3-dioxygenase and galectin-3 (IDO-Gal3) to shift tryptophan metabolism toward the kynurenine pathway in the joint. Here, we hypothesized intra-articular IDO-Gal3 would prevent osteoarthritis progression in male rats as assessed via measures of pain-like behavior, synovial fluid metabolite profile, and joint structure. Joint degeneration was induced by medial collateral ligament transection and full or partial transection of the medial meniscus. IDO-Gal3 was delivered by a single intra-articular injection 3-4 days later. In the first cohort of Sprague Dawley rats, medial tibial plateau cartilage area increased following IDO-Gal3 treatment compared to saline (vehicle) treatment at four weeks. Hind paw withdrawal threshold was increased compared to vehicle control for four weeks. In the second cohort of Lewis rats, synovial fluid metabolomic profiles measured by LC-MS were altered in IDO-Gal3-treated rats at one week and six weeks. At one week, tryptophan metabolism pathway was altered in IDO-Gal3-treated animals. Amino acid pathways involved in collagen turnover, such as arginine and proline, were downregulated at six weeks in injured rats treated with IDO-Gal3. Energy metabolism pathways followed a similar trend. There was no change in medial tibial plateau cartilage area or percent trabecular bone area at six weeks, nor were there differences in bone microstructure, likely related to the partial medial meniscus transection. In conclusion, a single intra-articular injection of IDO-Gal3 improved tactile sensitivity and resulted in short- and long-term shifts in synovial fluid metabolic profiles.

Indexed as

Indoleamine-Pyrrole 2,3,-DioxygenaseJoint InstabilityKnee JointMetabolomeSynovial FluidAnimalsInjections, Intra-ArticularMaleRatsRats, Inbred LewRats, Sprague-DawleyTryptophanIndoleamine-Pyrrole 2,3,-DioxygenaseTryptophanGalectin-3Indoleamine 2,3-dioxygenaseIntra-articular drug deliveryKnee osteoarthritisMetabolomics

Identifiers

PMID41862150
PMCPMC13215561

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