Evidence map›Paper›PMID 41861576›Full record

ArticleInternational dental journal2026

Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis.

Xuanhe Feng, Liang Hu, Chen Zhang, Wanzhen Lei, Bowen Zhou, Dengsheng Xia, Zhaochen Shan, Luyuan Jin

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PubMed Full text at PMC Publisher
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Article in International dental journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

8 authors.

Xuanhe FengEmergent Dental Care and General Dentistry, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Liang HuDepartment of Oral and Maxillofacial Surgery Clinic, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Chen ZhangEmergent Dental Care and General Dentistry, Beijing Stomatological Hospital, Capital Medical University, Beijing, China; Beijing Institute of Dental Research, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Wanzhen LeiEmergent Dental Care and General Dentistry, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Bowen ZhouDepartment of Oral and Maxillofacial Surgery Clinic, Beijing Stomatological Hospital, Capital Medical University, Beijing, China.
Dengsheng XiaEmergent Dental Care and General Dentistry, Beijing Stomatological Hospital, Capital Medical University, Beijing, China. Electronic address: xdsh1202@aliyun.com.
Zhaochen ShanDepartment of Oral and Maxillofacial Surgery Clinic, Beijing Stomatological Hospital, Capital Medical University, Beijing, China. Electronic address: shanzhch629@163.com.
Luyuan JinEmergent Dental Care and General Dentistry, Beijing Stomatological Hospital, Capital Medical University, Beijing, China. Electronic address: sujin_0309@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveWhile diabetes is known to impair dentin regenerative capacity, the specific role of endothelial nitric oxide synthase (eNOS) is poorly defined. This study aimed to elucidate how eNOS regulates the osteo/odontogenic differentiation of human dental pulp stem cells (hDPSCs) under high-glucose conditions and to determine its functional role in dentin regeneration in vivo.

methodsImmunohistochemistry and Western blot were used to analyse eNOS and odontogenic marker expression in diabetic dental pulp. hDPSCs with eNOS knockdown or overexpression were cultured under high glucose and evaluated using ALP activity, alizarin red staining, Western blot, and RT-qPCR. A rabbit tooth extraction model with DPSC implantation assessed in vivo dentin regeneration via micro-CT, histology, and electron microscopy.

resultsDiabetic pulp showed reduced eNOS, DSPP, and DMP1 expression. High glucose suppressed p-AKT/eNOS signalling and impaired hDPSC differentiation, which was rescued by eNOS overexpression. In vivo, eNOS-overexpressing cells promoted significantly more dentin-like tissue formation with elevated DSPP/DMP1 expression.

conclusionseNOS is essential for maintaining hDPSC differentiation under high-glucose conditions. Targeting eNOS signalling may represent a novel strategy to enhance dentin regeneration in diabetic patients. CLINICAL RELEVANCE: Targeting the AKT/eNOS signalling axis offers a novel therapeutic strategy to enhance dentin regeneration in diabetic patients.

Indexed as

Dental PulpDentinNitric Oxide Synthase Type IIIProto-Oncogene Proteins c-aktRegenerationAnimalsBlotting, WesternCell DifferentiationCells, CulturedDentin SialophosphoproteinExtracellular Matrix ProteinsGlucoseHumansImmunohistochemistryPhosphoproteinsRabbitsDentin SialophosphoproteinExtracellular Matrix ProteinsGlucoseNitric Oxide Synthase Type IIIPhosphoproteinsProto-Oncogene Proteins c-aktSialoglycoproteinsDiabeteseNOSHuman dental pulp stem cellsOdontogenic differentiation

Identifiers

PMID41861576
PMCPMC13018931

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.