Evidence map›Paper›PMID 41861112›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Embedded CRISPRi Enhances Gene-Silencing Efficiency in Drosophila.

Pengchong Fu, Xuedi Zhang, Ying Zhou, Jie Zheng, Angyang Sun, Kailong Zhuang, Weiwei Bao, Guanjun Gao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pengchong FuSchool of Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou, Jiangsu Province, China.
Xuedi ZhangSchool of Basic Medical Sciences, Suzhou Medical College, Soochow University, Suzhou, Jiangsu Province, China.
Ying ZhouGene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Jie ZhengGene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Angyang SunGene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Kailong ZhuangGene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Weiwei BaoGene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.
Guanjun GaoGene Editing Center, School of Life Science and Technology, ShanghaiTech University, Shanghai, China.ORCID https://orcid.org/0000-0002-3535-8681

Funding

National Natural Science Foundation of China 32370631National Natural Science Foundation of China 32500494priority academic program development of jiangsu higher education institutions
6 · The paper itself

Abstract

CRISPR interference (CRISPRi), leveraging catalytically inactive Cas9 (dCas9), has transformed transcriptional silencing. However, its application in Drosophila melanogaster has been constrained by inconsistent efficiency and limited repression amplitude. Here, we present embedded CRISPR interference (emCRISPRi), an advanced gene-silencing platform that integrates transcriptional repression domains (Mxi and TRD) into a structurally flexible region of dCas9. This design significantly enhances silencing efficiency, enabling robust repression of coding genes and cis-regulatory elements, particularly at transcription start site (TSS)-proximal regions. emCRISPRi demonstrates improved gene-silencing activity compared to RNA interference (RNAi) at several tested loci and facilitates strong phenotypic rescue via unmodified cDNA. Its versatility is demonstrated through the dissection of Hippo pathway interactions and the mitigation of TDP-43-induced neurotoxicity in an amyotrophic lateral sclerosis (ALS) model. These findings position emCRISPRi as a transformative tool for functional genomics, enhancer studies, and disease modeling in Drosophila, with significant potential for cross-species adaptation and therapeutic innovation.

Indexed as

Amyotrophic Lateral SclerosisCRISPR-Cas SystemsDrosophila melanogasterGene SilencingAnimalsDisease Models, AnimalDrosophila ProteinsRNA InterferenceDrosophila ProteinsCRISPRidCas9drosophilatranscriptional repression

Identifiers

PMID41861112
PMCPMC13588077

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.