Protocol to study murine ovarian elasticity and composition in situ by integrating quantitative micro-elastography with light microscopy.

Anna Jaeschke, Matt S Hepburn, Alireza Mowla, Hagen Eckert, Brendan F Kennedy, Chii Jou Chan

Abstract read

In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

6 authors.

Anna JaeschkeMechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Center for Neuromusculoskeletal Restorative Medicine, Hong Kong Science Park, Shatin, NT, Hong Kong SAR, China; School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Ma Liu Shui, NT, Hong Kong SAR, China.

Matt S HepburnBRITElab, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Department of Electrical, Electronic & Computer Engineering, School of Engineering, The University of Western Australia, Crawley, WA 6009, Australia; Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Toruń, Grudziądzka 5, 87-100 Toruń, Poland.

Alireza MowlaBRITElab, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Department of Electrical, Electronic & Computer Engineering, School of Engineering, The University of Western Australia, Crawley, WA 6009, Australia.

Hagen EckertDepartment of Mechanical Engineering and Materials Science, Duke University, 144 Hudson Hall, Durham, NC 27708, USA.

Brendan F KennedyBRITElab, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands and Centre for Medical Research, The University of Western Australia, Nedlands, WA 6009, Australia; Department of Electrical, Electronic & Computer Engineering, School of Engineering, The University of Western Australia, Crawley, WA 6009, Australia; Institute of Physics, Faculty of Physics, Astronomy and Informatics, Nicolaus Copernicus University in Toruń, Grudziądzka 5, 87-100 Toruń, Poland. Electronic address: brendan.kennedy@uwa.edu.au.

Chii Jou ChanMechanobiology Institute, National University of Singapore, Singapore 117411, Singapore; Department of Biological Sciences, National University of Singapore, Singapore 117558, Singapore. Electronic address: dbschii@nus.edu.sg.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Here, we present a protocol combining quantitative micro-elastography (QME) with transmitted light (TL) and immunofluorescence (IF) microscopy to study ovarian elasticity and composition in situ. We describe steps for excising murine ovaries, acquiring QME scans, and fixing the tissue. Subsequently, the tissue is sectioned and imaged with TL microscopy to locate functional components, such as corpora lutea and follicles, and segment them within the QME data. Finally, IF is used to analyze cell and molecular composition. For complete details on the use and execution of this protocol, please refer to Jaeschke et al.

Indexed as

Elasticity Imaging TechniquesMicroscopyOvaryAnimalsElasticityFemaleMiceMicroscopy, FluorescenceBiophysicsDevelopmental biologyMicroscopy

Identifiers

PMID41860871

DOI10.1016/j.xpro.2026.104434

PMCPMC13010434

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LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.