Evidence map›Paper›PMID 41860152›Full record

ArticleVeterinary medicine and science2026

Seroprevalence and Risk Factors of Cystic Echinococcosis in Cattle and Buffaloes: Insights From an In-House ELISA.

Mughees Aizaz Alvi, Talha Javaid, Majed H Wakid, Hong-Bin Yan, Muhammad Wasim Usmani, Li Li, Warda Qamar, Aliza Ali, Junaid Amjed, Wan-Zhong Jia and 1 more

Abstract read
In one paragraph

Article in Veterinary medicine and science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

11 authors.

Mughees Aizaz AlviState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID https://orcid.org/0000-0002-8800-2953
Talha JavaidDepartment of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.ORCID https://orcid.org/0009-0008-4177-0315
Majed H WakidDepartment of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Saudi Arabia.ORCID https://orcid.org/0000-0003-4941-5373
Hong-Bin YanState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID https://orcid.org/0000-0002-8522-0875
Muhammad Wasim UsmaniInstitute of Drug Discovery Technology, Ningbo University, Ningbo, Zhejiang, China.ORCID https://orcid.org/0000-0001-9065-2552
Li LiState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID https://orcid.org/0000-0001-6467-0247
Warda QamarDepartment of Parasitology, University of Agriculture, Faisalabad, Pakistan.ORCID https://orcid.org/0000-0001-6704-150X
Aliza AliDepartment of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.ORCID https://orcid.org/0009-0003-1879-8604
Junaid AmjedDepartment of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.ORCID https://orcid.org/0009-0000-2186-2150
Wan-Zhong JiaState Key Laboratory of Animal Disease Control and Prevention, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, China.ORCID https://orcid.org/0000-0002-0801-7478
Muhammad SaqibDepartment of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan.ORCID https://orcid.org/0000-0002-2796-1413

Funding

Central Public-Interest Scientific Institution Basal Research Fund 1610312023012Gansu Province Joint Research Fund 24JRRA807Innovation Program of Chinese Academy of Agricultural Sciences CAAS-ASTIP-2021-LVRIInnovation Program of Chinese Academy of Agricultural Sciences CAASTIP-2025-05Key Research and Development Program of Rikaze RKZ2025ZD-009NBCITS CARS-37The Major Science and Technology Project of Gansu Province 22ZD6NA001The Major Science and Technology Project of Gansu Province 23ZDNA007The Major Science and Technology Project of Gansu Province 24ZD13NA008
6 · The paper itself

Abstract

backgroundCystic echinococcosis (CE) is a zoonotic disease caused by the larval stage of Echinococcus granulosus and associated with productivity and economic losses in the livestock population across the world.

objectivesThis cross-sectional study spanned over a period of 1 year (2024) to determine the seroprevalence and associated risk factors of CE in large ruminants (cattle and buffaloes) hosted in different districts of Punjab, Pakistan, using an in-house developed ELISA.

methodsOverall, 430 serum samples from 197 buffaloes and 233 cattle were collected for detection of anti-E. granulosus antibodies using an in-house ELISA.

resultsThe highest seropositivity rate (68.24%) was in cattle, whereas buffaloes had 19.28%. The geographical variation revealed that the lowest seropositivity rate (9.52%) was in Haroonabad, whereas Bahawalpur had the highest seropositivity rate (96.29%). Sex-wise, females showed a seroprevalence of 46.13%, whereas males had a seroprevalence of 37.50%. Age-wise trends showed that seropositivity increased up to 10-12 years of age, while decreasing in older animals. Breed-wise, the highest seropositivity rate of 96.29% was observed in the Cholistani cattle, and the lowest rate of 25.25% was shown by Nili-Ravi buffaloes. The LASSO model revealed a high prediction accuracy of 76.98% and an area under the curve (0.73), suggesting geographical and host-related impacts on the epidemiology of CE. However, our findings indicate widespread seropositivity, the study does not evaluate production losses or economic impact, and our conclusion refers to epidemiological patterns.

conclusionsThere is a prompt need for targeted strategies under the One Health concept for region-specific surveillance, improving animal husbandry practices and controlling definitive hosts, particularly in high-risk areas of Pakistan.

Indexed as

BuffaloesCattle DiseasesEchinococcosisEchinococcus granulosusAnimalsCattleCross-Sectional StudiesEnzyme-Linked Immunosorbent AssayFemaleMalePakistanRisk FactorsSeroepidemiologic StudiesEchinococcus granulosusELISAlivestockOne HealthPakistanseroprevalence

Identifiers

PMID41860152
PMCPMC13098088

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.