Evidence map›Paper›PMID 41859925›Full record

ArticleThe Plant journal : for cell and molecular biology2026

Virus-induced gene editing of stomatal regulators in Nicotiana benthamiana enables rapid functional genomics.

Redeat Tibebu, Maria Elena Gamo, Evan E Ellison, Erik A Myers, Rishika Sahoo, Sydney R Winecke, Grace D Tan, James M Fischer, Andrew D B Leakey, Daniel F Voytas

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In one paragraph

Article in The Plant journal : for cell and molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

10 authors.

Redeat TibebuGraduate Program in Plant and Microbial Biology, University of Minnesota, St. Paul, Minnesota, USA.
Maria Elena GamoCenter for Precision Plant Genomics, University of Minnesota, St. Paul, Minnesota, USA.
Evan E EllisonCrop Science Centre, Department of Plant Sciences, University of Cambridge, Cambridge, CB3 OLE, UK.
Erik A MyersGraduate Program in Plant and Microbial Biology, University of Minnesota, St. Paul, Minnesota, USA.ORCID 0009-0003-3523-7307
Rishika SahooDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, USA.
Sydney R WineckeDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota, USA.
Grace D TanSchool of Integrative Biology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
James M FischerDepartment of Plant Biology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.
Andrew D B LeakeySchool of Integrative Biology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA.ORCID 0000-0001-6251-024X
Daniel F VoytasCenter for Precision Plant Genomics, University of Minnesota, St. Paul, Minnesota, USA.ORCID 0000-0002-4944-1224

Funding

U.S. Department of Energy SC0018277U.S. Department of Energy SC0023160
6 · The paper itself

Abstract

Virus-induced gene editing (VIGE) holds promise as a rapid and scalable approach for functional genomics in plants. Here, we apply a tobacco rattle virus (TRV)-based single-guide RNA (sgRNA) delivery system to target key regulators of stomatal development in Nicotiana benthamiana using transgenic Cas9-expressing lines. sgRNAs fused to a mobile RNA element and co-delivered with TRV enabled both somatic and heritable genome editing across orthologs of STOMAGEN, EPF2, YODA, and SPEECHLESS. Somatic editing frequencies reached up to 95%, and heritable tetra-allelic mutations were recovered in multiple target genes. Mutants exhibited significant, gene-specific changes in stomatal density, with corresponding effects on leaf temperature indicative of altered evaporative cooling. Additionally, sgRNAs fused to an AmCyan reporter enabled visualization of virus-infected tissues, allowing stomatal phenotyping in edited M

Indexed as

Gene EditingNicotianaPlant StomataPlant VirusesCRISPR-Cas SystemsGenomicsPlant ProteinsPlants, Genetically ModifiedPlant ProteinsCRISPRgene editingstomatavirus‐induced gene editing (VIGE)

Identifiers

PMID41859925
PMCPMC13003430

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.