Evidence map›Paper›PMID 41859375›Full record

ArticleKidney international reports2026

Albumin-Keratin Casts Obstruct Renal Tubular and Vascular Lumens Following Kidney Ischemia.

Martina Bryant, Olivia Boykin, Olivia Mosley, Angela Ajith, Oliver Oglesby, Konstantin Kurzin, Carlee Harris, Sadaf Husan, Daniela Dokam, Sarah R McLarnon and 7 more

Abstract read
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In one paragraph

Article in Kidney international reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

17 authors.

Martina BryantDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Olivia BoykinDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Olivia MosleyDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Angela AjithDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Oliver OglesbyDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Konstantin KurzinDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Carlee HarrisDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Sadaf HusanDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Daniela DokamDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Sarah R McLarnonDepartment of Cell Biology and Physiology, School of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA.
Jingping SunDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Brendan MarshallDepartment of Anatomy and Cell Biology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Amanda BarrettDepartment of Pathology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Aaron PolichnowskiDepartment of Biomedical Sciences, Quillen College of Medicine, East Tennessee State University, Johnson City, Tennessee, USA.
Wendy B BollagDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
David MattsonDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.
Paul M O'ConnorDepartment of Physiology, Medical College of Georgia, Augusta University, Augusta, Georgia, USA.

Funding

TRIO NRSA Training CoreTL1DK139567 · NIDDK · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Graca Duarte Almeida-Porada, Steven D Crowley · 2023 to 2026
$3.3M
NIDDK NIH HHS TL1 DK139567
6 · The paper itself

Abstract

Introduction: We have recently reported that in ischemic acute kidney injury (AKI), red blood cell (RBC) trapping causes fluid extravasation from the congested capillary circulation and subsequent toxic injury to the surrounding tubules. The goal of this study was to identify the cause of the obstruction leading to RBC trapping. Methods: Studies were performed in Sprague Dawley rats and human kidneys. Rats underwent warm arterial clamp ischemia for 45 minutes with 0 to 24 hours of reperfusion. New blood entering the kidney upon reperfusion of the kidney was tracked using Evans blue dye. At harvest, kidneys were fixed and histological analysis performed. Vascular and tubular casts from 4 human and 3 rat kidneys were isolated using laser dissection microscopy and their contents determined by mass spectrometry shotgun proteomics. Results: New blood entering the kidney appeared to be impeded in large renal veins by a homogeneous substance which stained blue in trichrome sections. Scanning electron microscopy revealed the substance to be a solid that conformed the shape of the encased RBCs. A similar substance was observed in the lumens of many tubules and small capillaries. Proteomic analysis identified albumin and keratins as major proteins present in the casts, and this was confirmed using immunohistochemistry. Conclusion: Our data indicate that albumin-keratin casts obstruct both vascular and tubular structures in the kidney following a period of ischemia. These casts may be the primary cause of vascular obstruction post-ischemia and likely lead to RBC trapping and associated acute tubular injury.

Indexed as

acute kidney injurycastscytokeratinischemia-reperfusionred blood cell trapping

Identifiers

PMID41859375
PMCPMC12996993

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