Evidence map›Paper›PMID 41859286›Full record

ArticleApplications in plant sciences

Just add water: A simple floral bud injection method for stable

Lauren E Stanley, David B Lowry

Abstract read
In one paragraph

Article in Applications in plant sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Lauren E StanleyDepartment of Plant Biology Michigan State University East Lansing Michigan USA.ORCID https://orcid.org/0000-0001-9579-0639
David B LowryDepartment of Plant Biology Michigan State University East Lansing Michigan USA.ORCID https://orcid.org/0000-0002-8182-1059

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Premise: Stable transformation is the biggest barrier to studying gene function in plants. In most species, transformation requires tissue culture and regeneration methods that may be arduous and cause undesirable genetic changes. Floral dip methods bypass these challenges by directly transforming ovules of developing flowers, but these methods have limited success outside of the Brassicaceae. Methods and Results: We demonstrate that floral dip methods are effective for some genotypes of the yellow monkeyflower, Conclusions: Through a combination of floral dip and injection methods, we transformed both coastal perennial and inland annual genotypes of

Indexed as

Agrobacteriumfloral transformationMimulus guttatusplant stable transformation

Identifiers

PMID41859286
PMCPMC12998258

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.