Electrochemical Dye Switching Assisted Spectral Demixing and 3D STORM Imaging.

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Article in Angewandte Chemie (International ed. in English), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0 citing papers in PubMed.

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5 · Who and what money

Authors and funding

3 authors.

Ying YangSchool of Chemistry and Australian Centre For NanoMedicine, University of New South Wales, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0001-6009-4748

Yuanqing MaSchool of Chemistry and Australian Centre For NanoMedicine, University of New South Wales, Sydney, New South Wales, Australia.

Justin GoodingSchool of Chemistry and Australian Centre For NanoMedicine, University of New South Wales, Sydney, New South Wales, Australia.ORCID https://orcid.org/0000-0002-5398-0597

Funding

Australian Research Council Discovery Grant Program DP250101047National Health and Medical Research Council GNT1196648

6 · The paper itself

Abstract

Super-resolution microscopy has advanced cellular imaging by enabling nanoscale resolution, while reliable multicolor strategies remain crucial for resolving multiple targets in complex cellular samples. Here, we demonstrate a two-color imaging strategy using electrochemically controlled stochastic optical reconstruction microscopy (EC-STORM), which allows precise control over the switching behavior of two cyanine dyes, Alexa 647 and CF680. An oscillating electrochemical potential enables control of emitter density and molecule ON time. This minimizes the spatial and temporal overlap of emitters, enabling effective spectral demixing for two-color EC-STORM imaging. We further combined spectral demixing with a biplane detection configuration, enabling additional 3D localization of Alexa 647, where axial coordinates are extracted from the photon-intensity ratio between the two detection channels instead of point spread function width. This integrated approach offers a practical solution for high-density multicolor 3D STORM imaging.

Indexed as

3D localization microscopyelectrochemical dye switchingmulticolor imagingspectral demixingsuper‐resolution microscopy

Identifiers

PMID41858166

DOI10.1002/anie.202517001

PMCPMC13110775

What OpenQuestion holds

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.