Evidence map›Paper›PMID 41855856›Full record

ArticleTranslational oncology2026

Ex vivo organotypic culture of liposarcoma effectively models in vivo supratherapeutic paclitaxel localized drug delivery.

Ilaria Caturegli, Rong Liu, Victoria Rivera, Olivia Taub, Mark W Grinstaff, Yolonda L Colson, Chandrajit P Raut

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Translational oncology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ilaria CaturegliDepartment of Surgery, Brigham and Women's Hospital, 75 Francis St., Boston, MA, 02115, USA. Electronic address: icaturegli@bwh.harvard.edu.
Rong LiuDivision of Thoracic Surgery, Department of Surgery, Mass General Brigham, 55 Fruit St., Boston, MA, 02114, USA.
Victoria RiveraDivision of Thoracic Surgery, Department of Surgery, Mass General Brigham, 55 Fruit St., Boston, MA, 02114, USA.
Olivia TaubDivision of Thoracic Surgery, Department of Surgery, Mass General Brigham, 55 Fruit St., Boston, MA, 02114, USA.
Mark W GrinstaffDepartment of Engineering, Boston University, 44 Cummington Mall, Boston, MA, 02215, USA.
Yolonda L ColsonDivision of Thoracic Surgery, Department of Surgery, Mass General Brigham, 55 Fruit St., Boston, MA, 02114, USA.
Chandrajit P RautDivision of Surgical Oncology, Department of Surgery, Mass General Brigham, 75 Francis St., Boston, MA, 02115, USA.

Funding

Supratherapeutic PTX Buttresses Reduce Locoregional Recurrence Rates Following Surgery for Soft Tissue SarcomasR01CA272637 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI Yolonda L Colson, MARK W. GRINSTAFF · 2022 to 2026
$3.5M
NCI NIH HHS R01 CA272637
6 · The paper itself

Abstract

backgroundRetroperitoneal sarcomas (RPS) exhibit a high locoregional recurrence rate after macroscopically complete surgical resection. Systemic therapies have limited efficacy and significant adverse effects. Sarcoma cell lines are susceptible to paclitaxel (PTX), a microtubule stabilizer, in 2-dimensional (2-D) monolayer cell culture, but resistant in animal models. When locally delivered via drug-eluting buttresses supratherapeutic concentrations are achieved and PTX becomes efficacious. Due to the limitations of 2-D culture, we establish an organotypic culture system to model the mechanisms by which supratherapeutic and prolonged exposure of PTX is effective.

methodsLiposarcoma tumors (LP6) were established subcutaneously in NU/J mice. Tumors were harvested, sliced with a vibratome (250 µm thick), and cultured on permeable trans wells.

resultsOrganotypic culture viability was maintained up to 7 days with greater than 50 % viability. Tumor slices were composed of 82 % ± 7 % human liposarcoma cells with the remainder being mouse stroma as determined by CD44 staining. Under 4-day exposure, IC

conclusionThis study reports a reproducible, clinically relevant organotypic culture liposarcoma model, which can serve as an intermediary between in vitro and in vivo studies.

Indexed as

Drug-eluting buttressesLiposarcomaLocalized drug deliveryOrganotypic culturePrecision medicine

Identifiers

PMID41855856
PMCPMC13014647

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.