Evidence map›Paper›PMID 41855680›Full record

ArticleBrazilian journal of otorhinolaryngology

Identification of hypoxia-related gene signatures and molecular subtypes in chronic rhinosinusitis with nasal polyps.

Lulu Song, Shican Zhou, Lili Wang, Ju Lai, Shiwang Tan, Chunyan Yao, Shaoqing Yu

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In one paragraph

Article in Brazilian journal of otorhinolaryngology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Lulu SongTongji University, School of Medicine, Tongji Hospital, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China; Tongji University, School of Medicine, Tongji Hospital, Department of Allergy, Shanghai, China.
Shican ZhouTongji University, School of Medicine, Tongji Hospital, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China; Tongji University, School of Medicine, Tongji Hospital, Department of Allergy, Shanghai, China.
Lili WangTongji University, Tongji Hospital, Department of Stomatology, Shanghai, China.
Ju LaiTongji University, School of Medicine, Tongji Hospital, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China; Tongji University, School of Medicine, Tongji Hospital, Department of Allergy, Shanghai, China.
Shiwang TanTongji University, School of Medicine, Tongji Hospital, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China; Tongji University, School of Medicine, Tongji Hospital, Department of Allergy, Shanghai, China.
Chunyan YaoTongji University, School of Medicine, Tongji Hospital, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China; Tongji University, School of Medicine, Tongji Hospital, Department of Allergy, Shanghai, China.
Shaoqing YuTongji University, School of Medicine, Tongji Hospital, Department of Otorhinolaryngology-Head and Neck Surgery, Shanghai, China; Tongji University, School of Medicine, Tongji Hospital, Department of Allergy, Shanghai, China. Electronic address: yu_shaoqing@163.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesChronic Rhinosinusitis with Nasal Polyps (CRSwNP) is a common and severe subtype of Chronic Rhinosinusitis (CRS), characterized by persistent inflammation and tissue remodeling in the nasal cavity. Hypoxia, resulting from chronic inflammation, is believed to play a pivotal role in CRSwNP pathogenesis. This study aims to identify Hypoxia-Related Genes (HRGs) involved in CRSwNP and explore their potential as diagnostic biomarkers and therapeutic targets.

methodsWe retrieved transcriptomic datasets (GSE136825 and GSE179265) from the Gene Expression Omnibus (GEO) database. Differential expression analysis was conducted to identify Hypoxia-Related Differentially Expressed Genes (HRG-DEGs) in CRSwNP. Machine learning techniques, including Least Absolute Shrinkage and Selection Operator (LASSO) regression and random forest, were employed to identify key HRGs. Validation of these genes was performed using qRT-PCR on clinical CRSwNP and control tissue samples. Additionally, consensus clustering analysis was applied to categorize CRSwNP patients into different molecular subtypes based on HRG expression, followed by gene ontology (GO) enrichment and immune infiltration analysis.

resultsA total of 19 hypoxia-related Differentially Expressed Genes (DEGs) were identified. Through LASSO regression and Random Forest analysis, followed by validation using an external dataset and qRT-PCR of clinical samples, four hypoxia-related hub genes ‒ CXCR4, HMOX1, DTNA, and FBP1 ‒ were identified. Receiver Operating Characteristic (ROC) curve analysis demonstrated that all four genes had Area Under the Curve (AUC) values exceeding 0.8. Additionally, consensus clustering revealed two distinct clusters with different hypoxia characteristics.

conclusionThis study identifies four key hypoxia-related genes (CXCR4, HMOX1, DTNA, and FBP1) that may serve as novel diagnostic biomarkers for CRSwNP. The molecular subtypes identified through clustering provide further insights into CRSwNP heterogeneity, suggesting the need for personalized treatment approaches targeting hypoxia-related pathways.

Indexed as

HypoxiaNasal PolypsRhinitisRhinosinusitisSinusitisTranscriptomeBiomarkersChronic DiseaseFemaleGene Expression ProfilingHumansBiomarkersBioinformaticsChronic rhinosinusitisHypoxiaNasal polyps

Identifiers

PMID41855680
PMCPMC13019074

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.