Evidence map›Paper›PMID 41854250›Full record

ArticlemSystems2026

Empowering global disease surveillance with CURED: a tool for rapid identification of unique genomic biomarkers.

Erin Theiller, Swetha Rajagopol, Stephanie Higgins, Dayanara I Torres, T'Nia Napper, Bianca E Galis, Arie Dash, Elizabeth Qian, Lauren Hamlette, Qianxuan She and 14 more

Abstract read
In one paragraph

Article in mSystems, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Erin TheillerCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.ORCID 0009-0001-4703-8925
Swetha RajagopolDivision of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Stephanie HigginsCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Dayanara I TorresCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
T'Nia NapperCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Bianca E GalisCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Arie DashCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Elizabeth QianDivision of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Lauren HamletteDivision of Gastroenterology, Hepatology, and Nutrition, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Qianxuan SheCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Ceylan TanesCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Nathan L'EtoileDivision of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.ORCID 0009-0006-1489-1686
Andries FederDivision of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Alice Slotfeldt VianaDepartamento de Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Matheus Assis Côrtes EstevesDepartamento de Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Michael C AbtDepartment of Microbiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.ORCID 0000-0002-7351-2061
Susan E CoffinDivision of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Ericka HayesDivision of Pediatric Infectious Diseases, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Robert F PotterCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.
Joseph P ZackularCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.ORCID 0000-0002-3228-3055
Lakshmi SrinivasanDepartment of Pediatrics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA.
Agnes Marie Sá FigueiredoDepartamento de Microbiologia Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil.
Paul J PlanetCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.ORCID 0000-0003-0543-0539
Ahmed M MoustafaCenter for Microbial Medicine, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA.ORCID 0000-0002-9949-6936

Funding

High Resolution, Whole Genome Tracking of Pathogen Transmission and Infection in the Neonatal ICU (NICU)R01AI185544 · NIAID · CHILDREN'S HOSP OF PHILADELPHIA · PI Lakshmi Srinivasan · 2025 to 2026
$1.8M
Bill and Melinda Gates Foundation INV-065400National Institute of Allergy and Infectious Diseases 1R01AI185544-01A1National Institute of Allergy and Infectious Diseases U19AI174998NIAID NIH HHS R01 AI185544
6 · The paper itself

Abstract

Rapid tracking of emerging pathogenic microorganisms is crucial for designing effective treatment, infection control, and prevention strategies. While whole-genome sequencing (WGS) offers the necessary granularity to track emerging clones, it remains prohibitively expensive at the scales needed to monitor with high resolution in real time. We present CURED (Classification Using Restriction Enzyme Diagnostics), which uses a training set of sequenced genomes to identify unique k-mers with restriction sites specific to a clonal lineage. CURED enables fast and inexpensive PCR-based diagnostic tests for surveillance or outbreak investigations with minimal use of WGS. Benchmarking against existing tools, CURED compares favorably and scales more efficiently than other k-mer search strategies. We validated and tested CURED in five distinct data sets: (i) previously identified biomarkers described for a methicillin-resistant

Indexed as

GenomicsAcinetobacter baumanniiBiomarkersClostridioides difficileGenetic MarkersGenome, BacterialGlobal HealthHumansMethicillin-Resistant Staphylococcus aureusPolymerase Chain ReactionWhole Genome SequencingBiomarkersGenetic Markersclonal lineage trackinghospitalinfection prevention and controlk-mer analysismicrobial diagnosticsoutbreakrapid pathogen surveillanceresource-limited settingsrestriction enzyme PCR

Identifiers

PMID41854250
PMCPMC13098284

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.