ArticleBiotechnology reports (Amsterdam, Netherlands)2026
Comparative analysis of anti-MICA scFv affinities: Insights from three label-free biophysical methods and biological validation.
Article in Biotechnology reports (Amsterdam, Netherlands), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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23 authors.
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Abstract
Antibody-antigen affinity is a key factor in the development of therapeutic antibodies, influencing candidate selection and their clinical efficacy. We used a combination of three label-free biophysical techniques: SPR, ITC, and fluorescence quenching, to rank anti-MICA single-chain variable fragments (scFvs), complemented by ELISA. We evaluated a wild-type (WT) scFv and its Beta mutant across the platforms, complementing this with molecular dynamics simulations and functional assays. The affinity constants (K
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