Diverse cultivation strategies are necessary to capture microbial diversity in High Arctic lake sediment.

Brittany M Berdy, Claire E Williams, Maria Sizova, Dawoon Jung, Nil Tandogan, Edgar D Goluch, Slava Epstein

Abstract read

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Article in Frontiers in microbiomes, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Brittany M Berdy *Broad Institute of MIT and Harvard, Cambridge, MA, United States.

Claire E Williams *Department of Biology, Northeastern University, Boston, MA, United States.

Maria SizovaDepartment of Biology, Northeastern University, Boston, MA, United States.

Dawoon JungNingbo Institute of Marine Medicine, Peking University, Ningbo, Zhejiang, China.

Nil TandoganDepartment of Chemical Engineering, Northeastern University, Boston, MA, United States.

Edgar D GoluchDepartment of Chemical Engineering, Northeastern University, Boston, MA, United States.

Slava EpsteinDepartment of Biology, Northeastern University, Boston, MA, United States.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

While metagenomics has revolutionized our understanding of microbial diversity and function, the cultivation of microorganisms remains indispensable for elucidating their physiological characteristics and potential biotechnological applications. Cultivation provides context to the vast metagenomic datasets and helps verify metagenome-based hypotheses on microbial interactions. The majority of microorganisms remain uncultivated, and this is particularly prominent from extreme environments such as the Arctic. Here we aimed to contribute to the growing body of work investigating microbial ecology in extreme environments by assessing the efficacy of a variety of cultivation approaches in lake sediment in the High Arctic. To try and capture the full breadth of organisms present, we used standard,

Indexed as

Arctic microbiologyin situ cultivationmicrobial cultivationmicrobial diversityuncultured microbiota

Identifiers

PMID41852418

DOI10.3389/frmbi.2025.1619859

PMCPMC12993673

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