ArticleAnalytical and bioanalytical chemistry2026
High-throughput microfluidic fluorescent aptasensor platform integrating dual carbon dots and Zn-TCPP for rapid detection of zearalenone.
Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Zearalenone (ZEN) is a mycotoxin widely present in crops, posing a serious threat to human health. Therefore, it is of great significance to establish a rapid and accurate method for zearalenone detection. In this work, a microfluidic device was produced using 3D printing technology and employed to construct a high-throughput fluorescence analysis platform. The blue fluorescent carbon quantum dots (B-CDs) and green fluorescent carbon quantum dots (G-CDs) were synthesized and incorporated into separate detection areas to achieve different signal responses. The B-CDs-cDNA/Apt1 and G-CDs-Apt2 complexes are pre-positioned in this area. The two-dimensional layered nanomaterial Zn-TCPP is used as an efficient quencher, achieving specific quenching by its adsorption on single-stranded DNA. Finally, high-throughput rapid detection was achieved by identifying RGB values via a smartphone. Under optimal conditions, the detection range of the fluorescent aptasensor is 0.5-500 ng/mL, with a limit of detection of 1.325 pg/mL. Moreover, this high-throughput fluorescent aptasensor demonstrates excellent analytical performance in the detection of real samples. This work provides a novel strategy for the rapid detection of zearalenone.
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