ArticleAntonie van Leeuwenhoek2026
Gluconic and ketogluconic acid production in Pseudomonas reptilivora B-6bs.
Article in Antonie van Leeuwenhoek, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Pseudomonas reptilivora B-6bs is a promising microbial platform for the industrial production of gluconic, 2-ketogluconic, and 5-ketogluconic acids. This study optimized the production of these metabolites via response surface methodology in a 3-L bioreactor. The experimental design evaluated the effects of the glucose concentration (10-50 g/L), aeration rate (0.2-2.0 vvm), and agitation speed (250-600 rpm). Metabolite concentrations were determined via specific colorimetric-spectrophotometric methods: the glucose oxidase method for glucose, the Malaprade-Hantzsch reaction for gluconic acid, o-phenylenediamine for 2-ketogluconic acid, and the arsenomolybdate method for 5-ketogluconic acid. The optimization results distinguished between model-predicted optima and experimentally observed outcomes. While the quadratic model suggested a theoretical maximum, the highest experimental efficiency was confirmed in T2 (30 g/L glucose, 600 rpm, and 2.0 vvm), which achieved specific conversion yields (Y
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