Evidence map›Paper›PMID 41850057›Full record

ArticlePoultry science2026

Evidence for lineage-associated differences in Tembusu virus pathogenicity in avian hosts.

Tao Wang, Yuyan Fan, Fan Liu, Wei Li, Yu He, Zhen Wu, Mingshu Wang, Renyong Jia, Rui Luo, Anchun Cheng and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Poultry science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Tao WangInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Yuyan FanInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Fan LiuInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Wei LiInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Yu HeInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Zhen WuInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Mingshu WangInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Renyong JiaInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China.
Rui LuoState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.
Anchun ChengInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Institute of Veterinary Immunology and Green Drugs, Veterinary Department in College of Animal Science, State Key Laboratory of Green Pesticide, Guizhou University, Guiyang, 550025, China.
Shun ChenInstitute of Veterinary Medicine and Immunology, Sichuan Agricultural University, Chengdu, Sichuan 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Agricultural Animal Diseases and Veterinary Public Health Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology for Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, 611130, China. Electronic address: shunchen@sicau.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Tembusu virus (TMUV) is a significant pathogen for waterfowl, yet the pathogenic differences among its evolutionary clusters remain unclear. This study systematically compared the pathogenic potential of TMUV isolates from key evolutionary branches (Clusters 2.1.1, 2.1.2, 2.2, 3.1 and 3.2) in both ducklings and chicks. Pathogenicity was assessed through in vitro replication kinetics, clinical disease, mortality, histopathology, viremia, and tissue viral loads. The results revealed host- and strain-specific pathogenicity. Duck-origin Cluster 2.1 strains were the most virulent in ducklings, while the chicken-origin Cluster 3 strain showed moderate pathogenicity. Notably, the Cluster 2.2 strain (MC) exhibited the highest replication efficiency in duck embryo fibroblasts (DEFs) but the lowest pathogenicity in vivo, indicating a disconnect between in vitro growth and in vivo virulence. All strains were weakly pathogenic in chicks. Furthermore, non-mosquito-borne transmission was demonstrated. This study elucidates the association between TMUV pathogenicity and its genetic evolution, underscores the critical role of host factors, and provides crucial insights for understanding its adaptive evolution and control.

Indexed as

ChickensDucksFlavivirusFlavivirus InfectionsPoultry DiseasesAnimalsPhylogenyVirulenceChicksDucklingsPathogenicityPhylogeneticsTembusu virus

Identifiers

PMID41850057
PMCPMC13011246

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.