Evidence map›Paper›PMID 41848947›Full record

ArticlePlant molecular biology2026

Changes in Alcohol dehydrogenase gene expression throughout strawberry fruit ripening process.

Daniel Bustos, David Alarcón-Muñoz, Francisca Arriaza-Rodríguez, Felipe Moraga, Gabriela Urra, Carolina Parra-Palma, Francisca Hormazabal-Abarza, Patricio Ramos, Luis Morales-Quintana

Abstract read
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In one paragraph

Article in Plant molecular biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Daniel BustosLaboratorio de Bioinformática y Química Computacional, Departamento de Medicina Traslacional, Facultad de Medicina, Universidad Católica del Maule, Talca, 3480094, Chile.
David Alarcón-MuñozLaboratorio de Bioinformática y Química Computacional, Departamento de Medicina Traslacional, Facultad de Medicina, Universidad Católica del Maule, Talca, 3480094, Chile.
Francisca Arriaza-RodríguezMultidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Región del Maule, Cinco Poniente #1670, Talca, Chile.
Felipe MoragaMultidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Región del Maule, Cinco Poniente #1670, Talca, Chile.
Gabriela UrraLaboratorio de Bioinformática y Química Computacional, Departamento de Medicina Traslacional, Facultad de Medicina, Universidad Católica del Maule, Talca, 3480094, Chile.
Carolina Parra-PalmaMultidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Región del Maule, Cinco Poniente #1670, Talca, Chile.
Francisca Hormazabal-AbarzaMultidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Región del Maule, Cinco Poniente #1670, Talca, Chile.
Patricio RamosPlant Microorganism Interaction Laboratory, Instituto de Ciencias Biológicas, Universidad de Talca, Talca, Chile. pramos@utalca.cl.
Luis Morales-QuintanaMultidisciplinary Agroindustry Research Laboratory, Instituto de Ciencias Biomédicas, Facultad de Ciencias de la Salud, Universidad Autónoma de Chile, Región del Maule, Cinco Poniente #1670, Talca, Chile. luis.morales@uautonoma.cl.

Funding

Agencia Nacional de Investigación y Desarrollo ATE220014Fondo Nacional de Desarrollo Científico y Tecnológico 1240771Fondo Nacional de Desarrollo Científico y Tecnológico 1250346Fondo Nacional de Desarrollo Científico y Tecnológico 3240463
6 · The paper itself

Abstract

Fruit aroma is a key determinant of organoleptic quality in strawberry (Fragaria × ananassa), with volatile esters being the major contributors to its characteristic scent. Alcohol dehydrogenases (ADHs) catalyze the conversion of aldehydes into alcohols, which serve as essential precursors for ester biosynthesis via alcohol acyltransferases (AAT). Despite their potential role in aroma formation, the molecular identity and functional properties of ADH family members in strawberry remain largely unexplored. Here, we present a comprehensive genome-wide analysis of the FaADH gene family in the cultivated strawberry genome. Eleven FaADH genes were identified and characterized based on gene structure, phylogeny, and promoter cis-element composition. Expression profiling across five fruit developmental stages revealed dynamic regulation of several isoforms, with FaADH5, FaADH8, and FaADH11 showing strong expression during early fruit development. These patterns were consistent with biochemical assays, which showed declining total ADH enzymatic activity as ripening progressed. Homology-based protein modeling and molecular docking predicted differential substrate affinities among isoforms, with FaADH5 and FaADH8 displaying favorable binding to 1-butanol and 1-hexanol. Molecular dynamics (MD) simulations further confirmed the structural stability of these interactions over time. The integration of expression, activity, and structural data supports a functional specialization within the FaADH family, with select isoforms likely contributing directly to the alcohol pool required for ester formation. These findings provide new insights into the molecular basis of aroma development in strawberry and highlight FaADH genes as potential targets for breeding or metabolic engineering aimed at improving fruit flavor.

Indexed as

Alcohol DehydrogenaseFragariaFruitGene Expression Regulation, PlantAmino Acid SequenceGene Expression ProfilingMolecular Docking SimulationMolecular Dynamics SimulationPhylogenyPlant ProteinsPromoter Regions, GeneticAlcohol DehydrogenasePlant ProteinsAlcohol dehydrogenase (ADH)Fruit ripeningMolecular dynamic simulationsStrawberryVolatile organic compound

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.