Evidence map›Paper›PMID 41848210›Full record

ArticleJournal of clinical laboratory analysis2026

The Application Value of Flow Cytometry-Based Peripheral Blood Leukocyte Classification in Screening for Acute Leukemia and Posttreatment Monitoring.

Penghua Yan, Lianfeng Wu, Xiangyang Lin, Hong Lu, Jingjing Qian

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical laboratory analysis, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Penghua YanDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Lianfeng WuDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Xiangyang LinDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Hong LuDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.
Jingjing QianDepartment of Clinical Laboratory, Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, China.

Funding

Key Laboratory of Clinical Laboratory Diagnosis and Translational Research of Zhejiang Province 2022E10022Science and Technology Plan Project of Wenzhou Municipality Y2020750
6 · The paper itself

Abstract

objectiveAssess the application value of flow cytometry (FCM) for peripheral blood leukocyte (PBL) classification in acute leukemia (AL) screening and monitoring.

methodsAnalyzed EDTA-K2-anticoagulated blood from 100 nonhematological patients, 42 newly diagnosed AL patients, and 50 posttreatment leukemia patients using FCM, blood cell analyzers (BCA), and manual microscopy.

resultsFCM and BCA showed significant positive correlations in classifying and counting neutrophils, eosinophils, basophils, lymphocytes, and monocytes (r = 0.994, 0.671, 0.946, 0.987, and 0.849, respectively; p < 0.01). A significant positive correlation was observed between FCM and manual methods in counting blast cells (r = 0.882, p < 0.01). Using microscopy as the reference standard and a cutoff value of ≥ 1% blast cells, FCM demonstrated 100% sensitivity, 85.7% specificity, and 97.8% accuracy in detecting blast cells. The type of peripheral blood blast cells provided by FCM showed good consistency with the clinical diagnosis of leukemia (Kappa value = 0.834). Additionally, a positive correlation was found between the detection rates of bone marrow and peripheral blood blast cells using FCM in posttreatment patients with leukemia (rs = 0.860, p < 0.01).

conclusionFCM-based PBL classification is precise and efficient for detecting blasts, providing rapid subtype identification. Its strong correlation with marrow findings underscores its clinical utility in AL screening and monitoring.

Indexed as

Flow CytometryLeukemiaLeukocytesAdolescentAdultAgedFemaleHumansLeukocyte CountMaleMiddle AgedSensitivity and SpecificityYoung Adultacute leukemiaflow cytometryperipheral blood leukocyte classificationscreening and monitoring

Identifiers

PMID41848210
PMCPMC13163947

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