Evidence map›Paper›PMID 41847194›Full record

ArticleFrontiers in microbiology2026

Bactericidal and antibiofilm activity of lactic acid bacteria-derived cell free extracts against dairy-associated spoilage and pathogenic bacteria.

Andressa Falqueto, Rafaela da Silva Rodrigues, Luana Virgínia Souza, Antônio Fernandes de Carvalho, Cinzia Caggia, Luiz Augusto Nero, Solimar Gonçalves Machado, Cinzia Lucia Randazzo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Andressa FalquetoInovaLeite - Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Rafaela da Silva RodriguesInsPOA - Laboratório de Inspeção de Produtos de Origem Animal, Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Luana Virgínia SouzaDi3A - Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Catania, Italy.
Antônio Fernandes de CarvalhoInovaLeite - Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Cinzia CaggiaDi3A - Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Catania, Italy.
Luiz Augusto NeroInsPOA - Laboratório de Inspeção de Produtos de Origem Animal, Departamento de Medicina Veterinária, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Solimar Gonçalves MachadoInovaLeite - Laboratório de Pesquisa em Leites e Derivados, Departamento de Tecnologia de Alimentos, Universidade Federal de Viçosa, Viçosa, MG, Brazil.
Cinzia Lucia RandazzoDi3A - Dipartimento di Agricoltura, Alimentazione e Ambiente, Università degli Studi di Catania, Catania, Italy.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study aimed to evaluate the antimicrobial and antibiofilm potential of 15 LAB strains using five types of LAB-derived preparations: cell-free supernatants (CFS), sonicated-inactivated cells (IC), their combination (ICS), and their neutralized variants (CFS N, ICS N) to identify the most effective strain-extract combinations for potential application as natural biocontrol agents in dairy systems. Antimicrobial activity was assessed through agar diffusion assays, growth and biofilm inhibition, and determination of minimum inhibitory (MIC) and bactericidal (MBC) concentrations. The extracts were further characterized by pH and organic acid profiles using high-performance liquid chromatography (HPLC), and their mechanisms of action were investigated through cellular leakage assays, time-kill kinetics, and scanning electron microscopy (SEM). Several LAB-derived extracts exhibited strong antagonistic and antibiofilm activity against both spoilage and pathogenic bacteria. Non-neutralized CFS and ICS showed pronounced bactericidal activity, confirming the central role of organic acids in microbial inhibition. Z-score ranking and leakage assays identified

Indexed as

bacterial membrane disruptionbiopreservativefood safetyfood spoilagegrowth inhibitionorganic acid profile

Identifiers

PMID41847194
PMCPMC12989577

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.