ArticleThe Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology2025
The Diagnostic Potential and Regulatory Mechanisms of miR-3180-3p in Metabolic Dysfunction-Associated Fatty Liver Disease with Respect to Inflammation and Oxidative Stress.
Article in The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
BACKGROUND/
aimsmiR-3180-3p has been linked to hepatocellular carcinoma progression, but its role and molecular mechanisms in metabolic dysfunction-associated steatotic liver disease (MASLD) are still unclear. The objective is to explore whether miR-3180-3p serves as a diagnostic biomarker for MASLD and elucidate its function in mediating hepatic inflammatory and oxidative stress responses. MATERIALS AND
methodsThis cross-sectional study included 117 patients with MASLD and 100 healthy controls. Serum and cellular RNA were extracted for real-time polymerase chain reaction analysis. In vitro experiments utilized HepG2 cells treated with free fatty acids (FFAs) to mimic MASLD-related stress conditions, evaluating miR-3180-3p expression levels and its effects on oxidative stress mark ers (malondialdehyde, superoxide dismutase) and inflammatory cytokines (tumor necrosis factor-α, interleukin-6 (IL-6), IL-1β). Target genes of miR-3180-3p were identified using bioinformatics and dual-luciferase assays.
resultsSerum miR-3180-3p levels were significantly higher in MASLD patients than controls, showing strong diagnostic value and potential as a biomarker. Multivariate analysis identified it, along with C-reactive protein (CRP) and high-density lipoprotein (HDL) cholesterol, as an independent risk factor for MASLD. Its expression positively correlated with triglycerides, low-density lipoprotein cholesterol, white blood cell count, CRP, Fibrosis-4, and oxidative stress markers (OA, PA), but negatively with HDL cholesterol. In FFA-treated cells, higher miR-3180-3p was linked to increased oxidative stress and inflammation. SKI, a direct target of miR-3180-3p, when silenced, reversed the protective effects of miR-3180-3p inhibition against FFA-induced cellular damage.
conclusionmiR-3180-3p is a promising diagnostic biomarker for MASLD and contributes to disease progression by enhancing hepatic inflammation and oxidative stress via targeting SKI. Cite this article as: Song K, Qi P. The diagnostic potential and regulatory mechanisms of miR-3180-3p in metabolic dysfunction- associated fatty liver disease with respect to inflammation and oxidative stress. Turk J Gastroenterol. 2026;37(3):310-321.
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