ArticleCell communication and signaling : CCS2026
Excessive reactive oxygen species attenuated IL-17 production in CD161
Article in Cell communication and signaling : CCS, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
Abstract
backgroundImmunological non-responders (INR), distinguished by profound immune dysfunction, fail to achieve immune reconstitution despite receiving antiretroviral therapy (ART). INR experience lower life expectancy and higher rates of morbidity and mortality. CD8+ T cells are crucial in controlling HIV progression. However, the function and underlying mechanisms of CD8+ T cells in immune reconstitution remain poorly elucidated.
methodsSingle-cell RNA sequencing (scRNA-seq) and TCR sequencing were employed to decipher the heterogeneity of CD8+ T cells from immunological responder (IR) and INR. Flow cytometry was utilized to assess the frequency and phenotype of CD161++ CD8+ T cells, the levels of cytoplasmic reactive oxygen species (cytoROS) and intracellular cytokines. qRT-PCR was performed to quantify the mRNA expression of crucial genes.
resultsscRNA-seq revealed a CD8+ T cell cluster defined by high KLRB1 (CD161) expression that was enriched in IR. The depletion of CD161++ CD8+ T cells observed in people living with HIV (PLWH) could not be restored by ART, and the expression of CD161++ CD8+ T cells was higher in IR compared to INR, which correlated with both CD4+ T cell counts and their functional status among PLWH on ART. CD161++ CD8+ T cells manifested less susceptibility to activation, senescence, and exhaustion, along with a unique metabolic signature characterized by upregulated lipid and amino acid, alongside downregulated levels of glycolysis, cytoplasmic reactive oxygen species (cytoROS), and mitochondrial mass. Mechanistically, lower levels of cytoROS maintained the homeostasis of IL-17 production in CD161++ CD8+ T cell subset by regulating FOS in IR.
conclusionOur findings underscore the association between the CD161++ CD8+ T cell subset and immune reconstitution, providing further insight into the molecular mechanisms underlying their decline during HIV infection. These novel insights offer potential targets for interventions aimed at improving immune reconstitution in PLWH on ART.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.