ArticleApplied microbiology and biotechnology2026
Transcriptomic insights into fruiting body malformations in Lentinula edodes.
Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Lentinula edodes (shiitake mushroom), the world's most cultivated edible fungus, frequently develops malformed fruiting bodies during commercial cultivation, leading to significant economic losses. To elucidate the molecular basis of these deformities, we performed comparative transcriptome analysis of four distinct malformation types-marginal depression (H), central protrusion (P), twinning (T), and irregularity (I)-versus normal controls (N1 ~ N4) under identical cultivation conditions. Our results revealed deformity-specific transcriptional signatures: the I vs. N4 and T vs. N3 phenotypes exhibited the most severe dysregulation (288 and 275 differentially expressed genes (DEGs), respectively), followed by P vs. N2 (185 DEGs), while H vs. N1 showed minimal changes (61 DEGs). Two DEGs (LENED_000547 and LENED_004546) were consistently identified across all four malformed types. They shared multiple conserved sequence motifs yet exhibited significant functional divergence, putatively acting as a nuclear-localized regulatory protein and a membrane-bound structural protein, respectively. Functional enrichment indicated downregulation of genes associated with structural molecule activity, nucleotide binding, and transferase activity, suggesting a fundamental compromise in cellular integrity and metabolism. Conversely, genes involved in carbohydrate metabolism, transmembrane transport, and hydrolase activity were upregulated, suggesting altered nutrient allocation and cell wall dynamics. Kyoto Encyclopedia of Genes and Genomes analysis further highlighted disruptions in fructose/mannose metabolism, glutathione metabolism, and glycerolipid pathways. Weighted gene co-expression network analysis uncovered phenotype-correlated modules. Environmental stressors likely exacerbated these responses, as evidenced by the induced expression of several stress-responsive genes. This study suggests that intrinsic transcriptomic dysregulation drives L. edodes malformations, offering valuable targets for precision breeding and cultivation management. KEY POINTS: • Comparative transcriptomics provide preliminary molecular insights into four morphologically defined shiitake malformation phenotypes • Shared DEGs reveal conserved molecular drivers of abnormal development • Results guide breeding and cultivation strategies for improved shiitake yield.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.