Evidence map›Paper›PMID 41843762›Full record

ArticleG3 (Bethesda, Md.)2026

A reference genome sequence for the exceptionally long-lived Great Basin bristlecone pine, Pinus longaeva.

David B Neale, Aleksey V Zimin, Constance I Millar, Patrick E McGuire, Jessica A Hosea, Edward Li, Daniela Puiu, Winston Timp, Steven L Salzberg

Abstract read
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In one paragraph

Article in G3 (Bethesda, Md.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

9 authors.

David B NealeDepartment of Plant Sciences, University of California Davis, Davis, CA 95616, United States.
Aleksey V ZiminDepartment of Biomedical Engineering and Center for Computational Biology, Johns Hopkins University, Baltimore, MD 21218, United States.
Constance I MillarUSDA Forest Service, Pacific Southwest Research Station, Vallejo, CA 94592, United States.
Patrick E McGuireDepartment of Plant Sciences, University of California Davis, Davis, CA 95616, United States.
Jessica A HoseaDepartment of Biomedical Engineering and Center for Computational Biology, Johns Hopkins University, Baltimore, MD 21218, United States.
Edward LiDepartment of Biomedical Engineering and Center for Computational Biology, Johns Hopkins University, Baltimore, MD 21218, United States.
Daniela PuiuDepartment of Biomedical Engineering and Center for Computational Biology, Johns Hopkins University, Baltimore, MD 21218, United States.
Winston TimpDepartment of Biomedical Engineering and Center for Computational Biology, Johns Hopkins University, Baltimore, MD 21218, United States.ORCID 0000-0003-2083-6027
Steven L SalzbergDepartment of Biomedical Engineering and Center for Computational Biology, Johns Hopkins University, Baltimore, MD 21218, United States.ORCID 0000-0002-8859-7432

Funding

Computational Methods for Genome Assembly, Transcript Assembly, and Variant DiscoveryR01HG006677 · NHGRI · JOHNS HOPKINS UNIVERSITY · PI SALZBERG, STEVEN L. · 2011 to 2025
$10.7M
Computational Methods for Microbial and Microbiome Sequence AnalysisR35GM130151 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Steven L. Salzberg · 2019 to 2026
$2.9M
National Science Foundation IOS-2432298NHGRI NIH HHS R01 HG006677NIGMS NIH HHS R35 GM130151NIH HHS R01-HG006677NIH HHS R35-GM130151Vaughn-Jordan Foundation
6 · The paper itself

Abstract

Great Basin bristlecone pine (Pinus longaeva), one of two species of bristlecone pine, the other being Rocky Mountain bristlecone pine (P. aristata), is endemic to the high Great Basin mountains in eastern California, Nevada, and Utah. It is the upper treeline forest tree in this region, found mostly between 2900 and 3600 m. The primary goal of this project was to generate a reference genome sequence for P. longaeva that, among its many possible applications, will serve as an important genetic resource to better understand the genetic mechanisms underlying its extreme longevity and its adaptation to the extreme environmental conditions where it is found. A combination of short-read and long-read sequences were generated from haploid megagametophyte and diploid needle tissues, respectively. A customized genome assembly approach was used to construct a highly contiguous 23.8-gigabase genome with a scaffold N50 size of 1.2 gigabases. The chloroplast and mitochondrial genomes were assembled separately into circular chromosomes with lengths of 120 kilobases and 8.68 megabases, respectively. While the number of disease resistance genes known as nucleotide-binding leucine-rich repeat receptors (NLRs) and larger-than-average telomere lengths relative to other conifers have been suggested as genetic mechanisms for controlling longevity, we did not find strong evidence for their involvement. Clearly further study is needed.

Indexed as

Genome, PlantPinusGenome, MitochondrialGenomicsMolecular Sequence Annotationannotationbristlecone pineconifergenome assemblyNLRPinus longaevatelomere length

Identifiers

PMID41843762
PMCPMC13233319

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.