Evidence map›Paper›PMID 41843375›Full record

ArticleScience China. Life sciences2026

Sgf29 regulates pluripotency by maintaining chromatin accessibility and promoting the expression of core transcription factors.

Yanling Qiu, Sisi Yin, Tianqi Cao, Tengteng Xu, Simiao Liu, Zihui Luo, Yitong Zhou, Haiying Liu, Junjiu Huang

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Article in Science China. Life sciences, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yanling QiuMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Sisi YinMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Tianqi CaoMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Tengteng XuMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Simiao LiuMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Zihui LuoMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Yitong ZhouMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Haiying LiuMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China.
Junjiu HuangMOE Key Laboratory of Gene Function and Regulation, State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou, 510275, China. hjunjiu@mail.sysu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The pluripotency state of embryonic stem cells (ESCs) is determined by both their epigenome and transcriptome. Sgf29, a subunit of the transcriptional co-activator complex SAGA, plays a critical role in a wide range of biological processes. However, whether and how Sgf29 mediates epigenetic modifications and pluripotency gene transcription is not fully understood. Herein, we found that Sgf29 knockout (KO) induced the differentiation of mouse ESCs (mESCs) by reducing H3K9ac and chromatin accessibility at the promoters and enhancers of key pluripotency genes. Interestingly, Sgf29 interacted with Oct4 and Nanog, but not Sox2, to co-regulate pluripotency genes. Sgf29 KO reduced the binding of Oct4 to the promoter and enhancer loci of Nanog and Klf4, leading to reduced expression of themselves and their downstream pluripotency-related genes. Furthermore, Sgf29 knockdown (KD) significantly reduced blastocyst rate and decreased H3K9ac at the blastocyst stage, leading to aberrant Oct4 and Nanog in the pre-implantation embryo. Together, these findings suggest that Sgf29 maintains chromatin accessibility and regulates the transcription of pluripotency-related genes by forming a functional complex with Oct4 and Nanog, playing a critical role in the regulation of pluripotent cell fate.

Indexed as

ChromatinPluripotent Stem CellsTranscription FactorsAnimalsCell DifferentiationEmbryonic Stem CellsGene Expression Regulation, DevelopmentalHistonesKruppel-Like Factor 4MiceMice, KnockoutMouse Embryonic Stem CellsNanog Homeobox ProteinOctamer Transcription Factor-3Promoter Regions, GeneticChromatinHistonesKlf4 protein, mouseKruppel-Like Factor 4Nanog Homeobox ProteinNanog protein, mouseOctamer Transcription Factor-3Pou5f1 protein, mouseTranscription Factorschromatin accessibilityNanogOct4pluripotencypre-implantation embryoSgf29

Identifiers

PMID41843375

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.