A CoOOH nanosheet-based colorimetric method for sensitive detection of alkaline phosphatase in biological samples.

Yijiang Li, Shaoxing Chen, Leijuan Huang, Biying Lin, Zhixiong Cai, Yuanfu Xie

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Article in Analytical and bioanalytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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6 authors.

Yijiang LiZhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, China.

Shaoxing ChenZhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, China.

Leijuan HuangZhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, China.

Biying LinZhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, China.

Zhixiong CaiCollege of Chemistry, Chemical Engineering and Environment, Minnan Normal University, Zhangzhou, 363000, China. czx1816@mnnu.edu.cn.ORCID http://orcid.org/0000-0002-6458-870X

Yuanfu XieZhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou, 363000, China. yuanfuxie@126.com.

Funding

Climbing Project of Doctor's Studio, Zhangzhou Affiliated Hospital of Fujian Medical University PDA202403Natural Science Foundation of Fujian Province 2023J011815

6 · The paper itself

Abstract

Alkaline phosphatase (ALP) is an important clinical biomarker, and rapid activity quantification is needed in routine diagnostics. Here, we report a CoOOH nanosheet-based colorimetric assay for ALP that uses a H₂O₂-free TMB chromogenic module. CoOOH nanosheets catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to produce the blue oxidized product, while ALP hydrolyzes ascorbic acid 2-phosphate (AAP) to generate ascorbic acid (AA), which reduces/decomposes CoOOH and suppresses the TMB signal, resulting in a distinct blue-to-colorless readout. Under optimized conditions, the absorbance response is linear with ALP activity from 0.01 to 25 mU mL

Indexed as

Alkaline PhosphataseCobaltColorimetryNanostructuresOxidesAscorbic AcidBenzidinesChromogenic CompoundsHumansHydroxidesLimit of DetectionOxidation-ReductionReproducibility of Results3,3',5,5'-tetramethylbenzidineAlkaline Phosphataseascorbate-2-phosphateAscorbic AcidBenzidinesChromogenic CompoundsCobaltcobalt hydroxideHydroxidesOxidesAlkaline phosphatase detectionColorimetric methodCoOOH catalysisTMB

Identifiers

PMID41843075

DOI10.1016/j.jcis.2022.11.001

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