Evidence map›Paper›PMID 41843000›Full record

ArticleTropical animal health and production2026

Growth and physiological regulations of discarded cocoa seed meal on mixed-bred rabbits in humid tropics.

Luke Chukwudi Ali, Ndubuisi Samuel Machebe, Bright Chigozie Amaefule, Augustine Emeka Ibeziako, Paulinus Ikenna Umeugokwe, Irechukwu Callistus Nwosu, Charity Nkechinyere Aba, Chekwube Maureen Ugwu, Onwuamaeze Gabriel Ugwu

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Article in Tropical animal health and production, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Luke Chukwudi AliDepartment of Animal Science, Faculty of Agricultural Sciences, University of Nigeria, Nsukka, Enugu State, 410001, Nigeria. aliluke230@gmail.com.ORCID http://orcid.org/0000-0001-6393-2217
Ndubuisi Samuel MachebeDepartment of Animal Science, Faculty of Agricultural Sciences, University of Nigeria, Nsukka, Enugu State, 410001, Nigeria.ORCID http://orcid.org/0000-0001-9603-8107
Bright Chigozie AmaefuleDepartment of Animal Science, Faculty of Agricultural Sciences, University of Nigeria, Nsukka, Enugu State, 410001, Nigeria.ORCID http://orcid.org/0000-0002-7382-5287
Augustine Emeka IbeziakoDepartment of Animal/Fisheries Science and Management, Enugu State University of Science and Technology (ESUT), PMB 01660, Agbani, Enugu State, Nigeria.ORCID http://orcid.org/0009-0000-2891-7310
Paulinus Ikenna UmeugokweDepartment of Animal Science, Faculty of Agricultural Sciences, University of Nigeria, Nsukka, Enugu State, 410001, Nigeria.ORCID http://orcid.org/0009-0005-3017-0718
Irechukwu Callistus NwosuDepartment of Animal/Fisheries Science and Management, Enugu State University of Science and Technology (ESUT), PMB 01660, Agbani, Enugu State, Nigeria.ORCID http://orcid.org/0009-0008-8236-732X
Charity Nkechinyere AbaDepartment of Animal Science, Faculty of Agricultural Sciences, University of Nigeria, Nsukka, Enugu State, 410001, Nigeria.ORCID http://orcid.org/0009-0007-8764-7278
Chekwube Maureen UgwuDepartment of Animal Science, Faculty of Agricultural Sciences, University of Nigeria, Nsukka, Enugu State, 410001, Nigeria.ORCID http://orcid.org/0009-0001-9747-6691
Onwuamaeze Gabriel UgwuFederal Ministry of Livestock Development, Office of the SGF, Three Arms Zone, Maitama, Abuja, Nigeria.ORCID http://orcid.org/0009-0006-6101-271X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Discarded cocoa seed meal (DCSM), a byproduct of cocoa processing, has potential as an unconventional feed resource. This study evaluated the effects of DCSM on growth performance, haematology, lipid profile, and liver function of mixed-bred rabbits reared in the humid tropics. Over an eight-week feeding trial, forty-eight mixed-bred male and female rabbits (initial mean weight: 1.45 ± 0.42 kg) were randomly allocated to four dietary levels of DCSM treatments (G): G1 (0%), G2 (15%), G3 (30%), and G4 (45%), in a 4 × 2 factorial arrangement within a completely randomized design (three replicates of four rabbits each). At the end of the trial, blood samples were collected from four rabbits per treatment group. Results showed that feed intake declined progressively with increasing levels of DCSM. Significant differences (p < 0.05) were recorded for male and female rabbit on feed intake, feed conversion ratio, and mortality. Increasing dietary DCSM caused significant (p < 0.05) elevations in white blood cells, red blood cells, haemoglobin (Hb), and packed cell volume. Male rabbits exhibited higher (p < 0.05) Hb, mean corpuscular volume, and mean corpuscular haemoglobin concentration than females. Lipid profiles varied significantly (p < 0.05) across treatments, with G2 showing higher total cholesterol, triglycerides, and low-density lipoprotein compared to G1 (control), G3, and G4. Liver metabolites showed marked effects (p < 0.05) on total and direct bilirubin, with G4 presenting higher values than other groups. Albumin and alanine aminotransferase concentrations were also higher (p < 0.05) in males. The findings indicate that DCSM alters haematological indices, lipid metabolism, and liver metabolites but should be included at low levels (15–30%) in rabbit diets to minimize adverse effects.

Indexed as

Animal FeedCacaoSeedsAnimal Nutritional Physiological PhenomenaAnimalsDietFemaleLiverMaleRabbitsRandom AllocationTropical ClimateBloodGrowth performanceHaematologyLipid profileLiver enzymesMale and female rabbit

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.