Evidence map›Paper›PMID 41841639›Full record

ArticlePlant biotechnology journal2026

Analysis of BpbHLH Gene Family Responsive to MeJA Signalling in Betula platyphylla Suk. and Functional Mechanisms of BpbHLH42/44 in Genetic Improvement and Triterpenoid Biosynthesis.

Ying Li, Linlin Xu, Jiale Cui, Siyao Wang, Yige Yuan, Changyixin Xiao, Xiaozhou Luo, Dmitry Baleev, Yaguang Zhan, Jing Yin

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In one paragraph

Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Ying LiKey Laboratory of Saline Alkali Vegetation Ecology Restoration, Northeast Forestry University, Harbin, Heilongjiang, China.
Linlin XuKey Laboratory of Saline Alkali Vegetation Ecology Restoration, Northeast Forestry University, Harbin, Heilongjiang, China.
Jiale CuiCollege of Life Science, Northeast Forestry University, Harbin, Heilongjiang, China.
Siyao WangHarbin Research Institute of Forestry Machinery, National Forestry and Grassland Administration, Harbin, Heilongjiang, China.
Yige YuanKey Laboratory of Saline Alkali Vegetation Ecology Restoration, Northeast Forestry University, Harbin, Heilongjiang, China.
Changyixin XiaoKey Laboratory of Saline Alkali Vegetation Ecology Restoration, Northeast Forestry University, Harbin, Heilongjiang, China.
Xiaozhou LuoCenter for Synthetic Biochemistry, State Key Laboratory of Quantitative Synthetic Biology, Shenzhen Institute of Synthetic Biology, Shenzhen Institute of Advanced Technology, Shenzhen, Guangdong, China.ORCID https://orcid.org/0000-0001-9808-6890
Dmitry BaleevAll-Russian Scientific Research Institute of Medicinal and Aromatic Plants, Moscow, Russia.
Yaguang ZhanKey Laboratory of Saline Alkali Vegetation Ecology Restoration, Northeast Forestry University, Harbin, Heilongjiang, China.
Jing YinKey Laboratory of Saline Alkali Vegetation Ecology Restoration, Northeast Forestry University, Harbin, Heilongjiang, China.ORCID https://orcid.org/0000-0002-0029-5393

Funding

Fundamental Research Funds for the Central Universities of Northeast Forestry University 2572021AW17Key Projects of Heilongjiang Provincial Natural Science Foundation ZD2023C004Longjiang Science and Technology Talent Spring Swallow Support Program CYCX24023National Natural Science Foundation of China 32371840
6 · The paper itself

Abstract

The basic helix-loop-helix (bHLH) transcription factor family regulates plant secondary metabolism, development and stress responses. Although triterpenoids such as betulinic acid (BA), betulin (BT) and oleanolic acid (OA) from Betula platyphylla Suk. are of pharmacological importance, the methyl jasmonate (MeJA)-induced regulation of IVa bHLHs remains unclear. A total of 131 BpbHLH genes were identified, and functional characterisation was performed for two IVa members, BpbHLH42 and BpbHLH44, in yeast, tobacco, birch cells and transgenic plants. Both enhanced triterpenoid biosynthesis but exhibited distinct specificities: BpbHLH42 primarily promoted OA accumulation by activating BpHMGR, BpSE1/3, BpW and BpY6, while BpbHLH44 favoured BT production through BpW, BpY6 and BpY11. Both indirectly regulated BpY9 via BpMYB21, forming a bHLH-MYB complex, and the MeJA repressor BpJAZ2 disrupted the BpbHLH42-MYB21 interaction. Transgenic birch lines further revealed divergent phenotypes: BpbHLH42 overexpression enlarged and smoothed leaves and increased OA levels, whereas BpbHLH44 enhanced alkali stress tolerance by elevating secondary metabolite accumulation. These results uncover the differential regulatory roles of BpbHLH42 and BpbHLH44 in triterpenoid biosynthesis and demonstrate their potential for metabolic engineering and genetic improvement in woody plants.

Indexed as

AcetatesBasic Helix-Loop-Helix ProteinsBetulaCyclopentanesOxylipinsPlant ProteinsTriterpenesBetulinic AcidGene Expression Regulation, PlantMultigene FamilyPentacyclic TriterpenesPlants, Genetically ModifiedSignal TransductionAcetatesBasic Helix-Loop-Helix ProteinsBetulinic AcidCyclopentanesmethyl jasmonateOxylipinsPentacyclic TriterpenesPlant ProteinsTriterpenesbHLH transcription factorsgene regulationMeJA inductiontriterpenoid biosynthesis

Identifiers

PMID41841639
PMCPMC13205763

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.