Evidence map›Paper›PMID 41841496›Full record

ArticleNucleic acids research2026

Mistranslation from an endogenous tRNA variant in human pan-genome cell lines.

Peter Rozik, Henry Moore, Jeremy T Lant, Kyle S Hoffman, Sarah K Schultz, Baasil Afzal, Patricia P Chan, Lauren E Flynn, Ilka U Heinemann, Todd M Lowe and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nucleic acids research, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Peter RozikDepartment of Biochemistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
Henry MooreDepartment of Biomolecular Engineering, Baskin School of Engineering & UCSC Genomics Institute, University of California Santa Cruz, Santa Cruz, CA 95064, United States.
Jeremy T LantDepartment of Biochemistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
Kyle S HoffmanBioinformatics Solutions Inc., Waterloo, Ontario, N2L 3K8, Canada.
Sarah K SchultzDepartment of Biochemistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
Baasil AfzalDepartment of Anatomy and Cell Biology, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
Patricia P ChanDepartment of Biomolecular Engineering, Baskin School of Engineering & UCSC Genomics Institute, University of California Santa Cruz, Santa Cruz, CA 95064, United States.
Lauren E FlynnDepartment of Anatomy and Cell Biology, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.
Ilka U HeinemannDepartment of Biochemistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.ORCID 0000-0003-1721-1667
Todd M LoweDepartment of Biomolecular Engineering, Baskin School of Engineering & UCSC Genomics Institute, University of California Santa Cruz, Santa Cruz, CA 95064, United States.ORCID 0000-0003-3253-6021
Patrick O'DonoghueDepartment of Biochemistry, The University of Western Ontario, London, Ontario, N6A 5C1, Canada.ORCID 0000-0002-9500-8640

Funding

A Unified Atlas of Dynamic tRNA FunctionR01HG006753 · NHGRI · UNIVERSITY OF CALIFORNIA SANTA CRUZ · PI LOWE, TODD MICHAEL · 2012 to 2022
$4.6M
Canada Research Chairs 232341CIHR 165985CIHR 185961CIHR 197789CIHR 197789Huntington Society of Canada Research ChairNatural Sciences and Engineering Research Council of Canada 04282Natural Sciences and Engineering Research Council of Canada 04776Natural Sciences and Engineering Research Council of Canada 04839Natural Sciences and Engineering Research Council of Canada 580214Natural Sciences and Engineering Research Council of Canada 580241Natural Sciences and Engineering Research Council of Canada 596289NHGRI NIH HHS R01 HG006753Ontario Ministry of Research and Innovation ER18-14-183Rare Disease Models and Mechanism Network
6 · The paper itself

Abstract

Human transfer RNA (tRNA) anticodon variants are a source of translation error. The tRNASerAGA-2-3 variant (G35A) occurs in 2% of the human population and causes mis-incorporation of serine at phenylalanine codons. Here, we developed a dual fluorescent reporter to quantify mis-incorporation levels in live human and murine cells and validated mistranslation by mass spectrometry. In β-lymphocytes from individuals in the 1000 genomes project, we confirmed the anticipated genotype of cells with A35 minor alleles, and tRNA sequencing demonstrated expression, C32 hypo-modification, and partial 5'-fragmentation of the endogenous mutant tRNASerAAA. Nanoparticle delivery of the fluorescent reporter confirmed serine mis-incorporation in the pan-genome cell lines. The data demonstrate that a natural genome-encoded human tRNA mutant causes mistranslation in cells derived from healthy individuals. Our findings have important implications for translation fidelity in humans and the application of missense suppressor tRNAs to medicine.

Indexed as

Protein BiosynthesisRNA, TransferAnimalsAnticodonCell LineGenome, HumanHumansMiceMutationSerineAnticodonRNA, TransferSerine

Identifiers

PMID41841496
PMCPMC12993449

What OpenQuestion holds

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LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.