Evidence map›Paper›PMID 41841330›Full record

ArticleChembiochem : a European journal of chemical biology2026

Transaminase and Norcoclaurine Synthase One-Pot Cascades Towards (1S)-Tetrahydroisoquinolines.

Jianxiong Zhao, Yeke Ni, Yu Wang, John M Ward, Jack W E Jeffries, Helen C Hailes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jianxiong ZhaoDepartment Chemistry, University College London, London, UK.
Yeke NiDepartment Chemistry, University College London, London, UK.
Yu WangDepartment Chemistry, University College London, London, UK.
John M WardDepartment of Biochemical Engineering, University College London, London, UK.
Jack W E JeffriesDepartment of Biochemical Engineering, University College London, London, UK.
Helen C HailesDepartment Chemistry, University College London, London, UK.ORCID https://orcid.org/0000-0001-5574-4742

Funding

Biotechnology and Biological Sciences Research Council BB/Y007972/1Engineering and Physical Sciences Research Council EP/P020410/1Engineering and Physical Sciences Research Council EP/W019132/1
6 · The paper itself

Abstract

Tetrahydroisoquinolines (THIQs) are an important group of alkaloids, and many of these natural products and non-natural analogues are biologically active. For this reason, concise stereochemical synthetic routes are of significant interest. One strategy has been to use the Pictet-Spenglerase enzyme norcoclaurine synthase (NCS) with an arylethylamine and aldehyde in a single step reaction, alternatively enzyme cascades have been developed. The use of a transaminase (TAm) potentially provides efficient access to the aldehyde component required in NCS cascades, but currently suffers from limited substrate tolerance, in part due to potential complexities with the formation of mixed products. Here we present the development of a TAm-NCS cascade using structurally diverse primary amines to form (1S)-6,7-dihydroxy-THIQs via the generation of, in particular, aliphatic aldehyde intermediates in situ. Application of the one-pot cascade with amines successfully generated the corresponding products in up to >99% enantiomeric excess and 83% isolated yields. The cascade was also extended with a tyrosinase to produce a 3-hydroxymethylene substituted THIQ.

Indexed as

Carbon-Nitrogen LigasesTetrahydroisoquinolinesTransaminasesAldehydesStereoisomerismAldehydesCarbon-Nitrogen Ligasesnorcoclaurine synthaseTetrahydroisoquinolinesTransaminasesbiocatalytic cascadenorcoclaurine synthasePictet–Spengler reactiontetrahydroisoquinolinestransaminase

Identifiers

PMID41841330
PMCPMC12993796

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.