Evidence map›Paper›PMID 41841227›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications.

Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M Blackburn, Dana V Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Nicole VoDivision of Nephrology, University of Washington School of Medicine, Seattle, Washington, USA.ORCID https://orcid.org/0000-0003-1349-8349
Lorena de OñateInnovative Genomics Institute, University of California Berkeley, Berkeley, California, USA.
Maximillian FrankDivision of Nephrology, University of Washington School of Medicine, Seattle, Washington, USA.
Xian HuCanCell, Institute of Clinical Medicine, Faculty of Medisin, University of Oslo, Oslo, Norway.
Sophie M BlackburnDivision of Nephrology, University of Washington School of Medicine, Seattle, Washington, USA.
Dana V FossInnovative Genomics Institute, University of California Berkeley, Berkeley, California, USA.
Jasmine VillegasDivision of Nephrology, University of Washington School of Medicine, Seattle, Washington, USA.
Kunica AsijaInnovative Genomics Institute, University of California Berkeley, Berkeley, California, USA.
Dirk HockemeyerInnovative Genomics Institute, University of California Berkeley, Berkeley, California, USA.
Hongxia FuDepartment of Medicine, University of Washington School of Medicine, Seattle, Washington, USA.
Raymond J MonnatInstitute for Stem Cell and Regenerative Medicine, University of Washington School of Medicine, Seattle, Washington, USA.
Ross C WilsonInnovative Genomics Institute, University of California Berkeley, Berkeley, California, USA.
Benjamin S FreedmanDivision of Nephrology, University of Washington School of Medicine, Seattle, Washington, USA.ORCID https://orcid.org/0000-0003-2228-7383

Funding

Cell specific delivery of novel therapies to enhance glomerular regeneration and repairUC2DK126006 · NIDDK · UNIVERSITY OF WASHINGTON · PI SHANKLAND, STUART JAMES · 2020 to 2024
$4.0M
Unveiling Functional Roles of Apical Surface Interactions Between Opposing Cell LayersR35GM149516 · NIGMS · UNIVERSITY OF WASHINGTON · PI Hongxia Fu · 2023 to 2026
$1.8M
A Human Organoid Model of Polycystic Kidney DiseaseR01DK117914 · NIDDK · UNIVERSITY OF WASHINGTON · PI FREEDMAN, BENJAMIN SOLOMON · 2018 to 2022
$1.8M
Utility of Human Organoids for Safety and Efficiency Evaluations of Genome Editing TherapeuticsU01AI176460 · NIAID · UNIVERSITY OF WASHINGTON · PI FREEDMAN, BENJAMIN SOLOMON · 2023 to 2025
$1.0M
Mechanoregulatory mechanisms of von Willebrand disease and thrombosisK25HL135432 · NHLBI · UNIVERSITY OF WASHINGTON · PI FU, HONGXIA · 2017 to 2021
$890k
NHLBI NIH HHS K25 HL135432NIGMS NIH HHS R35 GM149516NIH HHS K25HL135432NIH HHS R01DK117914NIH HHS R35GM149516NIH HHS U01AI176460NIH HHS U01DK127443NIH HHS UC2DK126006
6 · The paper itself

Abstract

Classical intracellular delivery methods such as transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type-specific programmability. We demonstrate that an innovative methodology called calcium shock (CaSh) dramatically improves particle delivery into single cells, colonies, and organoids, and enables programmable delivery (CaSh-Pro) into specific cell types within heterocellular populations. Calcium shock works by increasing endocytotic uptake while simultaneously disarming cell-cell junctions. CaSh-Pro further incorporates specific molecular targeting agents and amphiphilic peptides for preferential editing of different cell types. Calcium shock improves expression of plasmid, ribonucleoprotein, or adeno-associated viral vectors with minimal toxicity in intact organoids representing diverse lineages. CaSh and CaSh-Pro provide simple, versatile protocols for genome editing in complex systems, to enable biological discovery and therapeutic development.

Indexed as

CalciumGene EditingGene Transfer TechniquesHumansOrganoidsCalciumamphiphilic peptidescell‐cell junctionsCRISPRhuman organoidsRNP

Identifiers

PMID41841227
PMCPMC13073298

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.