Evidence map›Paper›PMID 41841123›Full record

ArticlePeerJ2026

Transcriptomic and metabolomic profiling reveals media- and host-dependent responses to

Wenxiu Liu, Tiansheng Zhang, Qian Wang, Zhunduo Li, Yanrui Bai, Han Xiao, Yan Wang, Ruihong Xiao, Liyu Tong, Yana Li and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Wenxiu Liu *The Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Tiansheng Zhang *Research and Translational Center for Immunological Disorders, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.
Qian WangThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Zhunduo LiThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Yanrui BaiThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Han XiaoThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Yan WangThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Ruihong XiaoThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Liyu TongThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.
Yana LiDepartment of Pathophysiology, School of Basic Medicine, Binzhou Medical University, Yantai, China.
Xueli QuResearch and Translational Center for Immunological Disorders, Yantai Affiliated Hospital of Binzhou Medical University, Yantai, China.
Xu ZhaoInstitute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu, China.
Zhengchao ZhangDepartment of Urology, Yantai Affiliated Hospital of Binzhou Medical University, The Second Clinical Medical College of Binzhou Medical University, Yantai, China.
Hui SunThe Second Hospital & Clinical Medical School, Lanzhou University, Lanzhou, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background/aims: Host-microbiota co-evolution maintains homeostasis Methods: Results: Metabolomics revealed medium-dependent remodeling of the exometabolome: BHI-derived supernatants were enriched in fatty acyls, glycerophospholipids, and pathways related to carbohydrate, energy, and lipid metabolism, whereas GAM-derived supernatants contained higher levels of sphingolipids and organic carbonic acids linked to purine and polyunsaturated fatty acid metabolism. Across four cell lines, DEGs induced by BHI-derived supernatants were mainly enriched in metabolic pathways, while GAM-derived supernatants more often engaged immune- and inflammation-related pathways. DEG overlap between cell types was limited, and KEGG enrichment and multivariate analyses supported cell type-specific transcriptional patterns. Functionally, GAM-derived supernatants significantly increased IL-6 and IL-8 secretion, whereas BHI-derived supernatants were more closely associated with changes in intracellular triglycerides and the lactate/pyruvate ratio in a cell-dependent manner ( Conclusion: Metabolomic, transcriptomic, and functional data demonstrate that microbial culture conditions and host cell identity critically shape

Indexed as

Culture MediaMetabolomicsTranscriptomeCell LineGene Expression ProfilingHEK293 CellsHumansMetabolomeMultiomicsCulture MediaBHI and GAM mediaCell line modelsGut-derived S. hominisMetabolomeRNA sequencing

Identifiers

PMID41841123
PMCPMC12989153

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.