Nature versus nurture: genetic background and media composition shape endothelial cell transcriptomes in vitro.

Flóra Demeter, Márta Lídia Debreczeni, Zsuzsanna Németh, Erika Kajdácsi, Zoltán Doleschall, László Cervenak

Abstract read

In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Flóra Demeter *Department of Internal Medicine and Hematology, Semmelweis University, Szentkirályi u. 46, Budapest, 1088, Hungary. demeter.flora@semmelweis.hu.

Márta Lídia Debreczeni *Department of Internal Medicine and Hematology, Semmelweis University, Szentkirályi u. 46, Budapest, 1088, Hungary.

Zsuzsanna NémethDepartment of Internal Medicine and Hematology, Semmelweis University, Szentkirályi u. 46, Budapest, 1088, Hungary.

Erika KajdácsiDepartment of Internal Medicine and Hematology, Semmelweis University, Szentkirályi u. 46, Budapest, 1088, Hungary.

Zoltán DoleschallDepartment of Internal Medicine and Hematology, Semmelweis University, Szentkirályi u. 46, Budapest, 1088, Hungary.

László CervenakDepartment of Internal Medicine and Hematology, Semmelweis University, Szentkirályi u. 46, Budapest, 1088, Hungary.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Primary endothelial cells (e.g., HUVEC) are essential in basic research and drug development, yet contradictory or poorly reproducible experimental results are common in the literature. We investigated whether such discrepancies arise from differences in culture media or donor genetic backgrounds using Agilent microarray transcriptomic analysis of three donor-derived HUVEC lines cultured in four distinct media (regarding basic composition, serum- and growth factor content). Genome-wide cluster analysis of 13,823 expressed genes revealed that samples clustered primarily by medium. Of the 2215 genes showing differential expression, 1697 varied across media, while only 805 were affected by donor differences. Correlation and principal component analysis confirmed that media composition had a strong impact on gene expression. Functional analysis of nine key endothelial functions showed predominantly medium-dependent effects, particularly on mitosis, angiogenesis, inflammation, and adhesion. The pivotal role of media composition in shaping HUVEC transcriptome may underlie inconsistencies across studies.

Indexed as

Culture MediaHuman Umbilical Vein Endothelial CellsTranscriptomeAngiogenesisCluster AnalysisGene Expression ProfilingGene Expression RegulationHumansPrincipal Component AnalysisCulture MediaCell culture mediaGenetic heterogeneityHuman umbilical vein endothelial cellIn vitroPrimary cellsTranscriptomics

Identifiers

PMID41840119

DOI10.1038/s41598-026-43732-0

PMCPMC13121701

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