Evidence map›Paper›PMID 41840086›Full record

ArticleApplied microbiology and biotechnology2026

Development of a high-throughput label-free method for enrichment of antimetabolite-resistant yeast strains using pico-litre agarose beads.

Sagarika B Govindaraju, Thibaut J Wenzel, Jack T Pronk, Rinke J van Tatenhove-Pel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Sagarika B GovindarajuDepartment of Biotechnology, Delft University of Technology, Van Der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Thibaut J WenzelDSM-Firmenich Taste, Texture and Health, Alexander Fleminglaan 1, 2613 AX, Delft, The Netherlands.
Jack T PronkDepartment of Biotechnology, Delft University of Technology, Van Der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Rinke J van Tatenhove-PelDepartment of Biotechnology, Delft University of Technology, Van Der Maasweg 9, 2629 HZ, Delft, The Netherlands. R.J.vanTatenhove-Pel@tudelft.nl.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antimetabolites, which are structural analogues of metabolites or carbon sources that exhibit toxicity by disrupting metabolism, can couple phenotypes of interest to growth and thereby facilitate the selection of relevant strains from large mutant pools generated by random mutagenesis. Toxicity and high costs of many antimetabolites provide incentives to minimise liquid volume during cultivation, while still capturing the genetic diversity in large mutant pools. This study aims to explore the use of high-throughput microbead-based cultivation and label-free enrichment of antimetabolite-resistant mutants. The generation of a large number (~ 10

Indexed as

Drug Resistance, FungalHigh-Throughput Screening AssaysMicrospheresSaccharomyces cerevisiaeCulture MediaMutationCulture MediaAntimetaboliteHigh-throughputLabel-free detectionMicrodropletsYeast

Identifiers

PMID41840086
PMCPMC12996017

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.