ArticleThe EMBO journal2026
A functional overlap between actively transcribed genes and chromatin insulator elements.
Article in The EMBO journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Loop extrusion by cohesin plays a role in enhancer-activated gene expression early in differentiation.Nature communications · 2026Article
- Cohesin bridging as a physical principle of enhancer-promoter communication.bioRxiv : the preprint server for biology · 2026Article
- Facilitators may represent a new class of regulatory elements.Current opinion in genetics & development · 2026Review
- Noncoding function of super enhancer derivedRNA biology · 2025Review
- Mechanisms of Globin Gene Regulation in Mammals.Annual review of genetics · 2025Review
- Pervasive RNA-binding protein enrichment on TAD boundaries regulates TAD organization.Nucleic acids research · 2025Article
- The characteristics of CTCF binding sequences contribute to enhancer blocking activity.Nucleic acids research · 2024Article
- Cooperative insulation of regulatory domains by CTCF-dependent physical insulation and promoter competition.Nature communications · 2024Article
- Noncoding function of super enhancer derived mRNA in modulating neighboring gene expression and TAD interaction.bioRxiv : the preprint server for biology · 2023Article
- Understanding fundamental principles of enhancer biology at a model locus: Analysing the structure and function of an enhancer cluster at the α-globin locus.BioEssays : news and reviews in molecular, cellular and developmental biology · 2023Review
- Hi-TrAC detects active sub-TADs and reveals internal organizations of super-enhancers.Nucleic acids research · 2023Article
- Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.Nature protocols · 2022Review
- preciseTAD: a transfer learning framework for 3D domain boundary prediction at base-pair resolution.Bioinformatics (Oxford, England) · 2022Article
- A gain-of-function single nucleotide variant creates a new promoter which acts as an orientation-dependent enhancer-blocker.Nature communications · 2021Article
- The mouse alpha-globin cluster: a paradigm for studying genome regulation and organization.Current opinion in genetics & development · 2021Review
Corrections and comments
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Authors and funding
17 authors.
Funding
Abstract
The mammalian genome is organised into large topologically associating domains (TADs) and smaller sub-TADs or enhancer-promoter loops, which may contribute to the regulation of gene expression. These dynamic structures arise, at least partly, via cohesin-mediated loop extrusion delimited by insulator elements. By studying the structure and function of the alpha-globin locus during erythroid differentiation, we have previously shown that the juxtaposition of the enhancers and promoters during this process partly depends on cohesin-mediated loop extrusion, which appears to be delimited by 12 largely convergently orientated CTCF boundary elements. To define the downstream boundary of the sub-TAD, we removed four CTCF sites in informative combinations. This showed that rather than CTCF insulators, it is the transcriptionally active alpha-globin gene that defines the downstream boundary of the sub-TAD. Further, insertion of actively transcribed fragments of the α-globin gene between the enhancers and native genes leads to a reduction in native α-globin expression and accumulation of cohesin at the insertion site. This highlights an overlap in the functional role of the fundamental elements of the genome.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.