Evidence map›Paper›PMID 41839968›Full record

ArticleScientific reports2026

Carboxy-terminal 24-amino-acid peptide of integral membrane protein 2A is produced in the heart and stimulates atrial natriuretic peptide release.

Tsukasa Osaki, Akikazu Mochiduki, Mitsuhiro Nishigori, Ken Shinmura, Takahiro Miyazaki, Emi Mishiro-Sato, Tetsuya Kadonosono, Kayoko Tamaki, Nobuaki Okumura, Toshifumi Takao and 2 more

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In one paragraph

Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Tsukasa OsakiDepartment of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan. tosaki@med.id.yamagata-u.ac.jp.
Akikazu MochidukiDepartment of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan.
Mitsuhiro NishigoriDepartment of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan.
Ken ShinmuraDepartment of Cardiology, Keio University School of Medicine, Shinjuku-Ku, Tokyo, 160-8582, Japan.
Takahiro MiyazakiDepartment of Cell Biology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan.
Emi Mishiro-SatoDepartment of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan.
Tetsuya KadonosonoDepartment of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan.
Kayoko TamakiDepartment of Cardiology, Keio University School of Medicine, Shinjuku-Ku, Tokyo, 160-8582, Japan.
Nobuaki OkumuraLaboratory of Biomolecular Analysis, Institute for Protein Research, Osaka University, Suita, Osaka, 565-0871, Japan.
Toshifumi TakaoLaboratory of Protein Profiling and Functional Proteomics, Institute for Protein Research, Osaka University, Suita, Osaka, 565-0871, Japan.
Kazuki SasakiDepartment of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan. k-sasaki@po.kyoundo.jp.
Naoto MinaminoDepartment of Molecular Pharmacology, National Cerebral and Cardiovascular Center Research Institute, Suita, Osaka, 564-8565, Japan. minamino@prf.or.jp.

Funding

Intramural Research Fund of the National Cerebral and Cardiovascular Center, Japan 22-1-4, 27-1-5, 30-1-12Japan Society for the Promotion of Science (JSPS) KAKENHI Grant-in-Aid for Young Scientists (B) JP23790888
6 · The paper itself

Abstract

Humoral factors regulating cardiac myocytes have not yet been fully elucidated. Using mass spectrometry, we analyzed peptides released from primary neonatal rat cardiac fibroblasts to identify a 24-amino-acid peptide with an intramolecular disulfide bond. It is derived from the C-terminal end of integral membrane protein 2A (ITM2A), a single-pass type II membrane protein; the peptide sequence is identical between humans and rodents and N-terminally flanked by conserved dibasic residues KR. The ITM2A peptide stimulated secretion of atrial natriuretic peptide (ANP), the predominant hormone produced by cardiac myocytes, in a Langendorff rat heart perfusion system as well as a primary culture of neonatal rat ventricular myocytes. These ANP secretion changes were not accompanied by an alteration in the beating rate, a key determinant of ANP secretion. In a mouse cardiac infarction model, both plasma ANP concentration and ITM2A gene expression in heart tissue reached a peak level on day 7 after operation. Our findings provide new insight into cardiac cell-to-cell communication that regulates ANP secretion.

Indexed as

Atrial Natriuretic FactorMembrane ProteinsMyocardiumAmino Acid SequenceAnimalsCells, CulturedFibroblastsMaleMiceMyocardial InfarctionMyocytes, CardiacRatsAtrial Natriuretic FactorMembrane ProteinsAtrial natriuretic peptideBiologically active peptideCardiac fibroblastCardiomyocyteIntegral membrane protein 2APeptidomics

Identifiers

PMID41839968
PMCPMC13125332

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.