ArticleZhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery2026
[Efficacy analysis of electrochemical detection of leukocyte esterase based on glucosyl ester in diagnosis of periprosthetic joint infection after knee arthroplasty in rabbits].
Article in Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Objective: To explore the diagnostic efficacy of electrochemical detection of synovial fluid leukocyte esterase (LE) based on glucosyl ester for periprosthetic joint infection (PJI) in rabbits. Methods: The enzyme kinetic parameters of the LE-catalyzed reaction based on glucosyl esters were determined electrochemically, and the charge-LE concentration relationship was plotted. Forty-eight healthy New Zealand rabbits were randomly divided into a sham-operation group (blank group), a knee joint metal prosthesis implantation group (control group), and a knee joint metal prosthesis implantation with PJI group (experimental group), with 16 rabbits in each group. The experimental group underwent intra-articular injection of 1×10 Results: The Michaelis constant of the enzymatic reaction of LE catalyzing glucosyl ester was 7.335 mmol/L, and the maximum reaction rate of the enzymatic reaction was 63.750 μmol·L Conclusion: Both synovial fluid LE and hematological testing exhibit good diagnostic efficacy in the rabbit PJI model, with the method based on electrochemical quantitative detection of glucose-based esters for LE demonstrating higher specificity, sensitivity, and accuracy.
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