ArticleBlood transfusion = Trasfusione del sangue
Lipidomic and metabolomic analysis of human erythrocytes during storage for transfusion applications.
Article in Blood transfusion = Trasfusione del sangue. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundThe preservation of concentrated red blood cells (RBCs) is crucial for their vitality and functionality. During storage, various metabolic changes occur, such as the loss of ATP, diphosphoglycerate, and potassium, as well as oxidative damage to proteins, lipids, and carbohydrates. Lipids, the most abundant class of metabolites in cells, are essential for maintaining the integrity and function of the red blood cell membrane. This study aims to evaluate molecular changes and identify potential biomarkers to optimise RBC transfusion by analysing metabolic and lipid profiles of preserved RBC at different time points. MATERIALS AND
methodsThirty samples from 10 freshly collected RBC units from 10 donors, were collected at three time points: collection, after 20 days, and after 40 days, and stored properly. The RBCs were then centrifugated, and the pellet was stored at -80°C for the metabolomic and lipidomic analyses using liquid chromatography coupled with mass spectrometry.
resultsLipidomic analysis revealed a progressive reduction in major membrane phospholipid classes, including phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, and phosphatidic acids. At increased resolution, molecular-species-level analysis identified selective depletion of arachidonate-containing phosphatidic acid species alongside a concomitant increase in free arachidonic acid, indicating active phospholipid remodeling during storage. Metabolomic profiling confirmed previously described alterations in glycolysis, energy metabolism, and redox-related pathways and revealed coordinated changes in metabolites linked to phospholipid synthesis and turnover. DISCUSSION: These findings confirm established features of RBC storage lesions while providing refined insight into membrane lipid remodeling at the molecular species and fatty acid building block levels. The integrated lipidomic and metabolomic approach highlights specific lipid signatures associated with RBC membrane aging, which may complement existing frameworks for studying storage-associated cellular changes and support future investigations linking molecular remodelling to functional RBC quality metrics.
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