Evidence map›Paper›PMID 41838299›Full record

ArticleMolecular biology reports2026

Early modulatory effects of Porphyromonas gingivalis and Lactobacillus salivarius on oncogenic processes in dysplastic oral keratinocytes.

Umme Samia, Tamara Vlajic Tovilovic, Ilinka Pecinar, Milena Radunovic, Sanja Petrovic, Milos Lazarevic, Dijana Mitic, Milica Jaksic Karisik, Nadja Nikolic, Jelena Carkic and 2 more

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Microorganisms · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Umme SamiaSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0009-0003-9781-5977
Tamara Vlajic TovilovicSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0002-3606-9646
Ilinka PecinarFaculty of Agriculture, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0002-5332-4696
Milena RadunovicSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0003-3507-1186
Sanja PetrovicSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0001-9493-8355
Milos LazarevicSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0003-1330-5332
Dijana MiticSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0002-0650-8193
Milica Jaksic KarisikSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0003-1114-0829
Nadja NikolicSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0002-8513-9313
Jelena CarkicSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia.ORCID http://orcid.org/0000-0002-1014-7589
Maria Cristina CuriaDepartment of Medical, Oral and Biotechnological Sciences, "G. d'Annunzio" University of Chieti-Pescara, Via dei Vestini, Chieti, 66100, Italy.ORCID http://orcid.org/0000-0003-4979-887X
Jelena MilasinSchool of Dental Medicine, University of Belgrade, Belgrade, 11000, Serbia. jelena.milasin@stomf.bg.ac.rs.ORCID http://orcid.org/0000-0002-6225-7210

Funding

Science Fund of the Republic of Serbia 750038
6 · The paper itself

Abstract

backgroundDisruption of oral homeostasis can contribute to diseases such as oral cancer. This study investigates the early modulatory effects of the probiotic Lactobacillus salivarius and the pathogen Porphyromonas gingivalis on dysplastic oral keratinocytes (DOK). METHODS AND

resultsDOK cells were co-cultured with each bacterium at a multiplicity of infection (MOI) of 10 for 2 and 6 h. Following co-culture, RNA was extracted to assess the relative expression of key carcinogenesis-related genes (DUSP16, PIK3CA, AKT, mTOR, ALOX5, VEGF, COX2, HEY1, JAGGED1) via qPCR. Complementary assays included Annexin V, scratch-wound healing, and Raman micro-spectroscopy to evaluate bacterial influence on DOK cell behavior and metabolism. Results showed significant downregulation of only two markers, mTOR and COX2, in the presence of L. salivarius, suggesting a mild antitumorigenic effect. Conversely, P. gingivalis induced marked upregulation of PIK3CA, AKT, ALOX5, and VEGF, consistent with pro-oncogenic activity. Functionally, P. gingivalis enhanced DOK cell migration at 6 h, while L. salivarius did not affect it. Additionally, L. salivarius-treated cells exhibited reduced reactive oxygen species (ROS) production compared to those exposed to P. gingivalis. Raman micro-spectroscopy further revealed distinct metabolic alterations between the two co-cultures.

conclusionsTogether, these findings indicate that L. salivarius may exert mild protective, anti-oncogenic effects on dysplastic oral keratinocytes, whereas P. gingivalis promotes oncogenic changes.

Indexed as

KeratinocytesLigilactobacillus salivariusMouth NeoplasmsPorphyromonas gingivalisCarcinogenesisCell MovementCoculture TechniquesCyclooxygenase 2HumansProbioticsTOR Serine-Threonine KinasesCyclooxygenase 2TOR Serine-Threonine KinasesDysplastic oral keratinocytesLactobacillus salivariusOral squamous cell carcinomaPIK3CA/AKT/mTORPorphyromonas gingivalisSignaling pathways

Identifiers

PMID41838299

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.