Evidence map›Paper›PMID 41838270›Full record

ArticleMolecular biology reports2026

First comprehensive molecular and phenotypic insights into multidrug resistance and virulence in poultry-associated Enterococcus spp. in Algeria.

Donia Mechoub, Sana Dhaouadi, Nacima Meguenni, Najwa Fatnassi, Madjid Akkou, Irene Marañón-Clemente, Carmen Torres, Ramzi Boubaker Elandoulsi, Yacine Titouche

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Article in Molecular biology reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Donia MechoubLaboratory of Analytical Biochemistry and Biotechnology (LABAB), University Mouloud Mammeri, Tizi Ouzou, Algeria.ORCID http://orcid.org/0009-0002-3576-3174
Sana DhaouadiBiotechpole Sidi Thabet, ISBST, BVBGR-LR11ES31, University of Manouba, Ariana, 2020, Tunisia.ORCID http://orcid.org/0000-0002-0595-2591
Nacima MeguenniLaboratory of Analytical Biochemistry and Biotechnology (LABAB), University Mouloud Mammeri, Tizi Ouzou, Algeria.ORCID http://orcid.org/0009-0005-8847-9638
Najwa FatnassiBiotechpole Sidi Thabet, ISBST, BVBGR-LR11ES31, University of Manouba, Ariana, 2020, Tunisia.
Madjid AkkouLaboratory of Animal Biotechnologies, Institute of Veterinary Sciences, University of Blida 1, Blida, Algeria.ORCID http://orcid.org/0000-0002-1631-9249
Irene Marañón-ClementeArea of Biochemistry and Molecular Biology, One Health-UR Research Group, University of La Rioja, Logroño, 26006, Spain.
Carmen TorresArea of Biochemistry and Molecular Biology, One Health-UR Research Group, University of La Rioja, Logroño, 26006, Spain.ORCID http://orcid.org/0000-0003-3709-1690
Ramzi Boubaker ElandoulsiBiotechpole Sidi Thabet, ISBST, BVBGR-LR11ES31, University of Manouba, Ariana, 2020, Tunisia.ORCID http://orcid.org/0000-0002-4161-9569
Yacine TitoucheLaboratory of Analytical Biochemistry and Biotechnology (LABAB), University Mouloud Mammeri, Tizi Ouzou, Algeria. yacine.titouche@ummto.dz.ORCID http://orcid.org/0000-0003-1062-5357

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundEnterococci are common members of the gastrointestinal microbiota of humans and animals, but can act as opportunistic pathogens with a strong ability to acquire and disseminate antibiotic resistance, representing a significant public health concern. Surveillance in poultry is essential to monitor the spread of resistant enterococci along the food production chain. This study aimed to characterize the antimicrobial resistance and virulence profiles of Enterococcus species isolated from poultry in Algeria. METHODS AND

resultsA total of 163 cloacal swabs were collected from broiler chickens, laying hens, and turkeys in breeding centers and slaughterhouses located in the provinces of Bouira and Tizi-Ouzou. Enterococci were identified using biochemical methods and MALDI-TOF MS. Antimicrobial susceptibility was assessed by disk diffusion, phenotypic traits were evaluated, and resistance and virulence genes were detected by PCR. Among 163 analyzed samples, 102 isolates (62.6%) were identified as Enterococcus spp., including E. faecium (61.8%), E. faecalis (31.4%), E. hirae (3.9%), and E. gallinarum (2.9%). High resistance rates were observed to tetracycline (96.1%), erythromycin (95.1%), and ciprofloxacin (64.7%). Multidrug resistance was detected in 96.1% of isolates. Biofilm formation was observed in 75.5% of strains, while 70.6% were slime producers. The most prevalent resistance genes were ermB (81.4%), tetM (55.9%), and tetK (49%). Virulence genes, including ace, efaA, gelE, asa1, hyl, and cylA, were variably detected.

conclusionsThe high prevalence of multidrug resistance and virulence determinants in poultry-associated enterococci highlights the need for strengthened antimicrobial stewardship, biosecurity measures, and surveillance to limit zoonotic transmission.

Indexed as

Drug Resistance, Multiple, BacterialEnterococcusAlgeriaAnimalsAnti-Bacterial AgentsChickensFemaleMicrobial Sensitivity TestsPhenotypePoultryPoultry DiseasesVirulenceVirulence FactorsAnti-Bacterial AgentsVirulence FactorsAlgeriaAntibiotic resistanceEnterococcusPoultryVirulence factors

Identifiers

PMID41838270

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.